Golden Nematode Program Manual - Phytosanitary Resources

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Procedures: Laboratory, Rack Room, and Wash Room Laboratory Sample Reading 7. Look for any round, spherical objects that appear to have a small spout or protrusion. Golden nematode cysts have a color range from golden-to shades of orange-to dark brown or black. See a microscopic view of golden nematode cysts in Figure 2-4-16 below. Photo by Zaphar Handoo FIGURE 2-4-16 Golden Nematode Cysts 8. If you locate a suspect golden nematode cyst, then continue to the instructions for removing cysts Step 5: Remove Cysts. 9. After you are finished examining the sample and have removed all suspect golden nematode cysts for identification, then dump the flotsam from the tray into the empty beaker. 10. Take the small #60 sieve, tri-section plastic tray, and reading well to the laboratory sink. Thoroughly clean each item, and make sure all material is removed. 11. Repeat each step above until all beaker samples have been examined. 2-4-28 Golden Nematode Program Manual 11/2007-01 PPQ
Procedures: Laboratory, Rack Room, and Wash Room Laboratory Sample Reading Step 5: Remove Cysts All golden cysts must be identified, whether they are considered to be viable or not. If you locate one or more golden nematode cysts when reading a sample, then do as follows: 1. Get one vial and a lid from the golden nematode station and take to the sampling station. A. Use only one vial per sample, even if there are multiple cysts from the same sample. B. Do not mix cysts from multiple samples into one vial. 2. Look under the microscope and use the loop to locate and isolate each golden nematode cyst. 3. Use the loop to remove the suspect golden nematode cyst(s) from the flotsam in the tray; and place each cyst into the vial. Screw on the lid. See Figure 2-4-17 below. Photo by Dan Kepich FIGURE 2-4-17 Golden Nematode Cyst Placement in Vial 11/2007-01 Golden Nematode Program Manual 2-4-29 PPQ
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United States Department of Agricul
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Contents 1 Golden Nematode Program
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List of Figures 1 Golden Nematode P
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List of Figures FIGURE A-1-14 Exam
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List of Tables 1 Golden Nematode Pr
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1 Introduction Golden Nematode Prog
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Introduction Users Users This manua
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Introduction Conventions Convention
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Introduction Conventions subordinat
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2 Procedures Golden Nematode Progra
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Procedures: Introduction Map of Qua
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Procedures: Introduction General Pe
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Procedures: Introduction General Pe
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2 Procedures Golden Nematode Progra
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Procedures: Preparation, Disinfecti
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Page 63 and 64: Procedures: Survey Surveys Outside
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Appendix A: Forms and Worksheets Go
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Appendix A: Forms and Worksheets Go
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Appendix A: Forms and Worksheets PP
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B Appendix Golden Nematode Program
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Appendix B: Fumigant Monitoring B.
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Appendix B: First Aid Rescue Breat
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C Appendix Golden Nematode Program
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Appendix C: Maintenance of the Manu
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Appendix C: Maintenance of the Manu
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Glossary 5 Golden Nematode Program
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Glossary: Definitions, Terms, and
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Index 1 C Canada 3-1-4 Carriers 2-2
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G Globodera rostochiensis (golden n
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Poisoning signs and symptoms B-1-6
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Trucks 3-1-17, 3-1-30 Tubers 3-1-3
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