Golden Nematode Program Manual - Phytosanitary Resources

Procedures: Laboratory, Rack Room, and Wash Room
Sample Wash Room
FIGURE 2-4-9 Sieves and Skimming Debris
8. As the larger debris is skimmed off with the running water, the
golden nematode cysts will float and come right up to the top
(you cannot see the cysts with the naked eye). Gently skim off
smaller debris (which may contain suspect nematode cysts) into
the top No. 20 sieve.
9. Thoroughly hose down the top screen and sieve sides to force
everything remaining in the top sieve down into the lower No. 60
sieve (bottom screen). This forces the smaller material down onto
the bottom sieve.
10. Each sample must process for a minimum of two minutes. At
this time you can start a second sample in the other can.
Do not mix the contents of the sample bags together.
Important
11. Thoroughly hose the bottom screen and force all material
(flotsam and cysts) from the bottom sieve into a beaker. Keep
adding water to the screen until the water level is about 2 inches
from the beaker’s top.
12. Remove the top screen from its holder and thoroughly clean the
screen, then replace in its holder.
13. Forcefully spray through the top screen into the bottom screen to
rinse all murkiness from the bottom (water running out of the
bottom screen should run clear)
14. Take the hose and add clear water into the beaker until the water
and flotsam is about 2" from the top.
15. Thoroughly clean the bottom screen, then return to its holder.
2-4-16 Golden Nematode Program Manual 11/2007-01
PPQ