Golden Nematode Program Manual - Phytosanitary Resources

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Procedures: Laboratory, Rack Room, and Wash Room Sample Wash Room Sample Wash Room Sample washing is the process by which suspect golden nematodes are removed from the soil. Conduct sample processing daily. Do not wash more samples than can be read within three hours of washing and before the work day’s end. Wash soil samples using a Fenwick can washer, also known as the soil sample washing machine. See Figure 2-4-5 below. Photo by Dan Kepich FIGURE 2-4-5 Fenwick Can Washers (Soil Sample Washing Machine) in Sample Wash Room Step 1: Gather Materials for Sample Washing Gather the following materials for sample washing: ◆ Beakers, empty and numbered1 through 50 (50) ◆ Bleach (hypochlorite) ◆ Bucket and mop ◆ Carts (2 flat surface, no hooks; each cart must be a different color) ◆ Lab sheet ◆ Pollen mask 2-4-10 Golden Nematode Program Manual 11/2007-01 PPQ
Procedures: Laboratory, Rack Room, and Wash Room Sample Wash Room ◆ Respirator ◆ Rubber apron ◆ Rubber gloves ◆ Safety glasses ◆ Sieve, No. 20 mesh (top sieve) ◆ Sieve, No. 60 mesh (bottom sieve) ◆ Tank There are two wash stations with two Fenwick cans each (total of 4 cans) for sample washing, so two people can process samples at a time. The collections must be kept separate during processing. Be careful not to cross-contaminate the samples during processing. Important Step 2: Prepare the Cleaning Solution A bucket of bleach solution is needed to mop the floor during the day. First thing each morning, prepare a bucket of disinfectant solution by mixing in the bucket: 1 cup of bleach to 3 gallons of hot water. Step 3: Wash Hands and Wear Proper Attire Wear the proper attire. Put a pollen mask, rubber apron, rubber gloves, and safety glasses on before entering the laboratory wash room. Important Some people choose not to wear rubber gloves during processing, due to sensitivity. If you do not wear gloves, then be sure to wash your hands before, during, and after sample washing. Wash your hands at the end of one collection and before starting a different collection. Wash your hands as follows: ◆ ◆ ◆ ◆ ◆ ◆ Before you begin sample washing Before putting on rubber gloves After emptying each sample bag After pushing sample bags down into trash bag After pushing the trash bag down into the trash can After closing and removing the trash bag from the trash can and wash room When wearing gloves, wash the gloves as follows: 11/2007-01 Golden Nematode Program Manual 2-4-11 PPQ
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United States Department of Agricul
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Contents 1 Golden Nematode Program
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List of Figures 1 Golden Nematode P
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List of Figures FIGURE A-1-14 Exam
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List of Tables 1 Golden Nematode Pr
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1 Introduction Golden Nematode Prog
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Introduction Users Users This manua
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Introduction Conventions Convention
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Introduction Conventions subordinat
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2 Procedures Golden Nematode Progra
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Procedures: Introduction Map of Qua
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Procedures: Regulatory Treatment an
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4 Procedures Golden Nematode Progra
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Procedures: Control Non-chemical Co
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A Appendix Golden Nematode Program
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Appendix A: Forms and Worksheets Go
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Appendix A: Forms and Worksheets We
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Appendix A: Forms and Worksheets PP
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B Appendix Golden Nematode Program
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Appendix B: Fumigant Monitoring B.
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Appendix B: First Aid Rescue Breat
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C Appendix Golden Nematode Program
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Appendix C: Maintenance of the Manu
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Appendix C: Maintenance of the Manu
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Glossary 5 Golden Nematode Program
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Glossary: Definitions, Terms, and
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Index 1 C Canada 3-1-4 Carriers 2-2
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G Globodera rostochiensis (golden n
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Poisoning signs and symptoms B-1-6
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Trucks 3-1-17, 3-1-30 Tubers 3-1-3
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Golden Nematode Program Manual - Phytosanitary Resources

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