Golden Nematode Program Manual - Phytosanitary Resources

Procedures: Laboratory, Rack Room, and Wash Room
Laboratory Sample Reading
Step 4: Read the Samples
Read the samples as follows:
1. Take the tri-section tray to the sample reading station.
2. Place the tray under the microscope.
3. Use the squirt bottle and slowly add water to the sample material
in each section of the tray so that any possible golden nematode
cysts will float to the top, but not flow over. All cysts will float,
whether viable (live) or non-viable (old, flattened, or dead).
4. Look into the microscope, and move the plastic sample tray
around until just a little bit of white plastic is showing at the
upper left top.
5. Start in the upper left corner, and use the probe to move the
sample material around to examine the sample.
Photos by Dan Kepich
FIGURE 2-4-15 Flotsam Examination for Cysts
6. Move the tri-section tray in small increments so that you see the
top white middle portion of the tray. Use a probe to move
material around and examine the sample. Continue moving the
tray and probing the material until you have examined the entire
sample in each section of the tray.
11/2007-01 Golden Nematode Program Manual 2-4-27
PPQ