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As it is generally found when the initial level<br />

of available nitrogen is low, the addition of a<br />

small amount of nitrogen reduced both the<br />

protein content .of the flour and the loaf volume.<br />

These two parameters, after reaching a<br />

minimum with the application of about 50 kg<br />

of nitrogen per hectare, increased with higher<br />

rates of fertilization in a nearly linear fashion<br />

up to the 300 kg/ha rate. Above this level of<br />

ferti Iization the protein percentage conti nued<br />

to increase while the loaf volume remained<br />

unchanged.<br />

Sedimentation volume of the flour from the<br />

two varieties was affected similarly by nitrogen<br />

fertilization. The first increment of nitrogen<br />

fertilization reduced the sedimentation volume<br />

from 43 to 33 cc; hligher rates gradually increased<br />

the sedimentation volume to a maximum<br />

of 63 cc with the application of 400 kg<br />

of nitrogen per hectare. The effect of nitrogen<br />

fertilization on the general strength index of<br />

the flour was very similar to the effect on<br />

protein percentage.<br />

Plant samples were taken from each experimental<br />

plot at 36, 67, 95 and 126 days after<br />

planting. The second sampling was made at<br />

heading and the last at physiological maturity.<br />

Except at the first sampling, the plants were<br />

divided into leaf, stem and spike tissue. The<br />

samples were washed, dried, ground and analyzed<br />

for total nitrogen, phosphorus, and<br />

potassium.<br />

The effect of nitrogen fertil ization on the<br />

nitrogen percentage in the wheat stems at<br />

67, 95 and 126 days after planting is shown in<br />

Fig. 2. At the two earlier sampling dates, the<br />

application of 100 kg of nitrogen per hectare<br />

clearly reduced the nitrogen percentage in<br />

the stems. Higher rates of nitrogen fertilization<br />

produced a logarithmic type increase in<br />

the nitrogen percentage. At 67 days after<br />

planting, the nitrogen percentage in the stem<br />

was increased from 1.13 to 2.25 by the application<br />

of 400 kg of nitrogen per hectare.<br />

The effect of nitrogen fertilization on the<br />

nitrogen percentage in the wheat leaves and<br />

spikes was similar to the effect on the stems.<br />

A reduction in the nitrogen percentage with<br />

the application of 100 kg of nitrogen per hectare<br />

was noted in the leaf at 95 and 126 days,<br />

and in the grain at 126 days. Logarithmic or<br />

linear increases in the nitrogen percentage of<br />

both leaves and spikes were observed at<br />

higher rates of nitrogen fertilization at all sam-<br />

Plant analyses of the wheat samples are carried out in the soil fertility laboratory of the Graduate College<br />

at Chapingo. Total nitrogen is determined by the micro-Kjeldahl method, which is shown here at<br />

distillation stage.

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