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As it is generally found when the initial level<br />
of available nitrogen is low, the addition of a<br />
small amount of nitrogen reduced both the<br />
protein content .of the flour and the loaf volume.<br />
These two parameters, after reaching a<br />
minimum with the application of about 50 kg<br />
of nitrogen per hectare, increased with higher<br />
rates of fertilization in a nearly linear fashion<br />
up to the 300 kg/ha rate. Above this level of<br />
ferti Iization the protein percentage conti nued<br />
to increase while the loaf volume remained<br />
unchanged.<br />
Sedimentation volume of the flour from the<br />
two varieties was affected similarly by nitrogen<br />
fertilization. The first increment of nitrogen<br />
fertilization reduced the sedimentation volume<br />
from 43 to 33 cc; hligher rates gradually increased<br />
the sedimentation volume to a maximum<br />
of 63 cc with the application of 400 kg<br />
of nitrogen per hectare. The effect of nitrogen<br />
fertilization on the general strength index of<br />
the flour was very similar to the effect on<br />
protein percentage.<br />
Plant samples were taken from each experimental<br />
plot at 36, 67, 95 and 126 days after<br />
planting. The second sampling was made at<br />
heading and the last at physiological maturity.<br />
Except at the first sampling, the plants were<br />
divided into leaf, stem and spike tissue. The<br />
samples were washed, dried, ground and analyzed<br />
for total nitrogen, phosphorus, and<br />
potassium.<br />
The effect of nitrogen fertil ization on the<br />
nitrogen percentage in the wheat stems at<br />
67, 95 and 126 days after planting is shown in<br />
Fig. 2. At the two earlier sampling dates, the<br />
application of 100 kg of nitrogen per hectare<br />
clearly reduced the nitrogen percentage in<br />
the stems. Higher rates of nitrogen fertilization<br />
produced a logarithmic type increase in<br />
the nitrogen percentage. At 67 days after<br />
planting, the nitrogen percentage in the stem<br />
was increased from 1.13 to 2.25 by the application<br />
of 400 kg of nitrogen per hectare.<br />
The effect of nitrogen fertilization on the<br />
nitrogen percentage in the wheat leaves and<br />
spikes was similar to the effect on the stems.<br />
A reduction in the nitrogen percentage with<br />
the application of 100 kg of nitrogen per hectare<br />
was noted in the leaf at 95 and 126 days,<br />
and in the grain at 126 days. Logarithmic or<br />
linear increases in the nitrogen percentage of<br />
both leaves and spikes were observed at<br />
higher rates of nitrogen fertilization at all sam-<br />
Plant analyses of the wheat samples are carried out in the soil fertility laboratory of the Graduate College<br />
at Chapingo. Total nitrogen is determined by the micro-Kjeldahl method, which is shown here at<br />
distillation stage.