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problem is due to aluminum toxicity. Additional<br />
laboratory and field studies are needed<br />
to explain the effects and interactions among<br />
phosphorus, lime, and silicate.<br />
Results of the 1969 studies clearly indicated<br />
that local varieties were poorly adapted to<br />
high levels of productivity. This fact was reflected<br />
in a significant increase in the stoner:<br />
grain ratio of the harvest from plots receiving<br />
chicken manure. A systematic collection of<br />
varieties from ecologically similar areas was<br />
made in cooperation with the Graduate School<br />
at Chapingo in early 1970, and these varieties<br />
are being tested at high levels of productivity.<br />
As a part of the study of Andosols, arrangements<br />
are being made for the publication<br />
of a review of the Japanese experience<br />
with this type of soil. Dr. Yoshiaki Ishizuka,<br />
Hokkaido University, Sapporo, Japan,<br />
has agreed to serve as coordinator-editor for<br />
the monograph. Drs. Tomoharu Egawa, Hideo<br />
Okajima and Akira Tanaka of Hokkaido University<br />
and Dr. Shinobu Yamada of Obihiro<br />
University have agreed to contribute chapters<br />
on "Properties of Andosols", "Amelioration of<br />
Andosols", "Maintenance of Fertility and Fertilizer<br />
Use" and "Distribution and Morphology<br />
of Andosols". It is expected that the manuscript<br />
will be prepared and edited by the end<br />
of 1970.<br />
METHODOLOGICAL<br />
RESEARCH<br />
Research is being continued to determine<br />
more efficient procedures for agronomic field<br />
trials and for interpreting experimental results.<br />
Dr. Foster B. Cady, University of Kentucky, is<br />
participating in the methodological studies.<br />
Visual symptoms of plant wilting as an index<br />
of moisture stress<br />
In rainfed agriculture, available soil moisture<br />
is an uncontrolled production factor that<br />
may vary significantly from plot to plot and<br />
contribute to a greatly inflated experimental<br />
error.<br />
To adjust plot yields for random differenences<br />
in the effect of moisture stress, this variable<br />
must be characterized at the plot level<br />
on many dates during the growing season.<br />
Visual symptom of wilting in corn, have been<br />
measured on individual plots and used as a<br />
covariate to adjust for differences in moisture<br />
stress within an experiment. These visual<br />
symptoms of wilting provide a measure of<br />
moisture stress that can be estimated rapidly<br />
and economically where labor costs are low.<br />
The degree of wilting in a plot of corn was<br />
estimated by counting the numbers of slightly<br />
and severely wilted plants. These numbers<br />
were then expressed as fractions of the total,<br />
with the fractions multiplied by empirical factors<br />
and summed to give the drought coefficient.<br />
These daily coefficients for each plot were<br />
transformed to a single value (the drought<br />
index) by multiplying by empirical factors and<br />
summing the products over the entire growing<br />
season. Empirical factors were estimated<br />
from the experiences of many investigators<br />
and reflect the relative importance of stress<br />
on yield at different physiological stages of<br />
growth.<br />
Since several observers of the same plot<br />
at the same time do not provide identical readings<br />
on wilting, an independent estimate was<br />
made of variance due to the observer. A<br />
group of observers was selected and the same<br />
group of observers took readings on the degree<br />
of wilting within each experimental plot.<br />
The daily drought coefficients were calculated<br />
from these readings, and the variance of these<br />
coefficients due to observers was calculated.<br />
This variance was used to calculate the variance<br />
due to observer, or measurement error,<br />
for the drought index.<br />
Before using drought indices as a covariate<br />
in the analysis of yield data, a preliminary test<br />
was made to determine whether the variation<br />
in drought indexes among plots was significantly<br />
greater than the measurement error.<br />
The overall mean squared deviation of the<br />
drought indices for the plots in an experiment<br />
was calculated. As this variance is a measure<br />
of the true variability among drought indices<br />
for the different plots, plus the variance<br />
due to observer, the F value (overall mean<br />
squared deviation) -+- (variance due to measurement)<br />
was calculated. A significant F value<br />
was interpreted to mean that the true variability<br />
in the drought indices among plots was<br />
greater than measurement error, thus the<br />
drought indices could be used as a covariate.<br />
The next step in evaluating drought index<br />
for use as a covariate was to measure the<br />
degree of association between the drought<br />
index and the experimental treatments and<br />
blocks. This association was measured by<br />
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