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Optimum Sample Size for Determining<br />
Stem Borer Incidence<br />
An arbitrary sample, which may be inaccurate<br />
and inefficient, is used frequently in<br />
chemical studies to estimate stem borer Infestations.<br />
In the experiments reported here,<br />
attempts ~ were made to estimate optimum<br />
sampl$ size, utilizing the data secured from<br />
three tests conducted at Paza Rica, Veracruz.<br />
There were a total of 68 plants in each<br />
plot. Experiment 1 obtained estimates on<br />
the effectiveness of several granular insecticide.<br />
Experiment 2 obtained estimates for<br />
timing applications of granular insecticides;<br />
While experiment 3 obtained estimates of the<br />
number of granular applications needed<br />
for effective control of borers, as well as fall<br />
armyworm. Damage records (number of Injured<br />
internodes per plant) were obtained<br />
from all plants within each plot. For the statistical<br />
analysis, random samples of four plants<br />
were combined in a stepwise manner to form<br />
the plot sizes. studied.<br />
Table 27 summarizes the results of the<br />
analyses. The data show that the experimental<br />
error mean square increased as the sample<br />
size increased. Also, the sampling error mean<br />
square (except in experiment 2) Increased<br />
slightly as the sample size increased.<br />
The stability of the sampling error indicates<br />
the homogeniety of the samples taken in each<br />
experimental plot. The reduction of the estimated<br />
variance of the mean permits estimation<br />
of the optimum sample size for experiments of<br />
this kind.<br />
It can be observed (Table 27), that the<br />
variance of the mean is considerably larger<br />
for a sample of 4 plants than for a sample<br />
of 48 plants. However, it also becomes obvious<br />
from the three tests, that the variance of<br />
the mean becomes stabilized for samples of<br />
24 plants or more. Thus, it may be considered<br />
that any increment of a sample size above 24<br />
plants will not result in a substantial reduction<br />
in the variance of the mean. The variance of<br />
the mean is important because it generates<br />
the standard error of mean.<br />
The estimation of treatment means is one<br />
of the basic objectives of the experiments,<br />
hence as the standard error of the mean becomes<br />
smaller, a greater precision is obtained<br />
in the comparisons of treatments. Finally, this<br />
information suggests that for experiments such<br />
as those mentioned above, 24 plants per plot<br />
(taken at random) is the most efficient sample<br />
size.<br />
TABLE 27. Statistics for estlmatlnS! optimum sample size for stem borer incidence in chemical control<br />
field trials. Poza Rica, Veracruz, Mexico, 1970.<br />
No." "ant.<br />
lnlpaCted<br />
Experiment No. 1 Experlmant No. 2 Experiment No.3<br />
EMS. EMS. 5·-<br />
X<br />
EMS. EMS.<br />
SI_<br />
Sz_<br />
EMS" EMS.<br />
X X<br />
41 7.49 3.70 0.031 9.17 2.99 0.031 3.16 2.59 0.016<br />
44 7.51 3.71 0.034 10.23 2.94 0.046 4.03 2.58 0.018<br />
40 7.35 3.69 0.037 9.06 2.93 0.045 3.48 2.57 0.022<br />
36 5.67 3.59 0.031 8.14 2.99 0.045 2.87 2.61 0.016<br />
32 4.30 .65 0.027 8.04 3.00 0.050 2.98 2.55 0.019<br />
2. 4.59 3.45 0.033 5.77 2.96 0.041 2.79 2.51 0.020<br />
24 4.24 3.47 0.035 6.29 3.00 0.052 2.19 2.53 0.018<br />
20 4.36 3.44 0.044 6.57 3.05 0.066 2.56 2.49 0.026<br />
16 5.33 3.59 0.067 5.12 3.18 0.064 2.48 2.64 0.031<br />
12 3.55 3.33 0.059 4.19 3.02 0.070 1.54 2.35 0.026<br />
•<br />
2.59 3.25 0.065 3.60' 2.66 0.090 1.17 2.16 0.029<br />
4 3.15 3.36 0.157 2.11 3.00 0.106 1.34 2.05 0.067<br />
EMS. = Experimental error mean square.<br />
_5. = Sampling error meen square. E.periment No. I: 6 tr.atment., 5 replication•.<br />
Experiment No.2: 5 treatment., 5 replication•.<br />
5·- = Varlanca of the meen.<br />
X<br />
Experiment No.3: 6 treetment., 5 replication•.<br />
NOTE:<br />
The number of In/urec:l Internod.. per plant wa. the criterium usec:l In determ'nlng the aHici.ncy of the dlffer.nt .ample .i•••.<br />
42