SUNDAY, APRIL 10 Across Societies – Experimental Biology

SUNDAYB87 517.3 Comparison of two fl uorescence-based assaysto a sensitive SDS-PAGE method for detection of trace proteasesin bovine serum albumin. E. Lang, L. Harwick, S. Stamenova,K. Rupprecht and J. Fishpaugh. Abbott Diagnostics Div.B88 517.4 Hydrohpobic interactions in an engineeredthreonine protease. M. Lansang and T. Baird, Jr. San FranciscoState Univ.B89 517.5 Antitumor activity ofrapamycin in combination with ectopic expression of Beclin 1in multidrug-resistant cancer cells. M. Lee, J-H. Ahn, K-H. Eumand Y-K. Kim. Univ. of Incheon, South Korea.B90 517.6 Carboxypeptidase O is an intestinal peptidasewith unique substrate specifi city. P.J. Lyons and L.D. Fricker.Albert Einstein Col. of Med.B91 517.7 Expression of recombinant human neutrophilelastase in Kluyveromyces lactis. E.T. Smith, J. Pugh, H.Klimecki and D.A. Johnson. East Tennessee State Univ.Quillen Col. of Med.B92 517.8 Ceruloplasmin binds and inactivates matrixmetalloproteinase-2. M.W. Thompson, J.P. Johnson and H.G.Abu-Taleb. Middle Tennessee State Univ.518. PROTEIN SYNTHESIS IN ORGANELLESPosterSUN. 7:30 AM—WASHINGTON CONVENTION CENTER, EXHIBITHALL ABCPresentation time: 12:25 PM-1:55 PMB93 518.1 Mammalian mitochondrial ribosomal proteins:revisited. B. Kumcuoglu, H. Cimen, N. Abu, M.E. Haque, L.L.Spremulli, H. Koc and E.C. Koc. Penn State and Univ. of NorthCarolina at Chapel Hill.519. PROTEIN TURNOVER IN CELL REGULATIONPosterSUN. 7:30 AM—WASHINGTON CONVENTION CENTER, EXHIBITHALL ABCPresentation time: 12:25 PM-1:55 PMB94 519.1 Induction of autophagic fl ux by amino aciddeprivation is distinct from nitrogen starvation-inducedmacroautophagy. H. Abeliovich, N. Ecker and D. Journo.NINDS, NIH and Hebrew Univ. of Jerusalem.B95 519.2 Functional characterization of Nedd4.1 andNedd4.2 HECT domain ligases. A.C. Augustus-Wallace, J.M.Klein and A.L. Haas. LSU Hlth. Sci. Ctr., New Orleans.B96 519.3 ClpXP modulates cell division in E. coli by amechanism that involves ATP-dependent degradation. J.L.Camberg, J.R. Hoskins and S. Wickner. NCI, NIH.B97 519.4 Molecular chaperone heat shock protein 90stabilizes nucleolin to maintain mRNA level in mitosis. J-J.Hung. Natl. Cheng Kung Univ., Taiwan.B98 519.5 Inactivation of eukaryotic initiation factor 5Aby specifi c acetylation of its hypusine residue by spermidine/spermine acetyltransferase 1. E.C. Wolff, S.B. Lee, J.H. Parkand M.H. Park. NIDCR, NIH.B99 519.6 The role of a ubiquitin processing proteasein the growth-to-development transition of Dictyosteliumdevelopment. H. Yamagata, N. Will, C. Parker and D. Lindsey.Walla Walla Univ.520. PROTEOLYTIC ENZYMES AND INHIBITORSPosterSUN. 7:30 AM—WASHINGTON CONVENTION CENTER, EXHIBITHALL ABCPresentation time: 12:25 PM-1:55 PMBIOCHEMISTRYB100 520.1 Testing the shifting specifi city model for enzymecatalysis. B.M. Britt. Texas Woman’s Univ.B101 520.2 Novel putative intracellular substrates orproducts for neurolysin in mice brain. L.M. Castro, C.C. C.Mendes, V. Oliveira and E.S. Ferro. Fed. Univ. of São Pauloand Univ. of São Paulo.B102 520.3 Utilizing a protein splicing side reaction togenerate a fragment for expressed protein ligation. S.L. Chin,A.C. Nadelson, D.J. York, J.N. Reitter and K.V. Mills. Col. ofthe Holy Cross, MA.B103 520.4 Evaluating the potency of HIV-1 proteaseinhibitors in blocking the initial step of HIV Gag-Pol processing.K. Davidoff, E. Soule, S. Daniels, D. Davis and R. Yarchoan.NCI, NIH.B104 520.5 Effects of the neuropeptidase thimetoligopeptidase on testicular development and testosteroneproduction. T. Dhawan, J.W. Qiao, D. Selen, L.A. Bruce, M.J.Tetel and A.J. Wolfson. Wellesley Col.B105 520.6 Determining the activation barrier and pHdependenceof each step of protein splicing. C.A. Donahue,D.J. York, D.M. Dorval, J.N. Reitter and K.V. Mills. Col. of theHoly Cross, MA.B106 520.7 Programmed cell death in the fi lamentousfungus Schizophyllum commune. K.M. Fox, A. Major, M.Richter and Z. Zeliang. Union Col., NY.B107 520.8 Proteolytic processing of Hendra virus fusionprotein in cells of the bat reservoir host. L. Lampe and R.E.Dutch. Univ. of Kentucky Col. of Med.B108 520.9 Conformational change analysis of thimetoligopeptidase. Z.J. Lin, J.A. Sigman and M.J. Glucksman.Saint Mary’s Col. of California and Rosalind Franklin Univ. ofMed. and Sci.B109 520.10 The infl uence of conserved catalytic residueson the mechanism of protein splicing of the Pyrococcus abyssiPolII intein. M.D. Marieni, L.R. Duffee, D.J. York, D.M. Dorval,J.N. Reitter and K.V. Mills. Col. of the Holy Cross, MA.B110 520.11 Non-canonical inteins: alternate mechanismsfor protein splicing. K.V. Mills, T.L. Powers, M.J. Drago, K.R.Connor and J.N. Reitter. Col. of the Holy Cross, MA.B111 520.12 Triple effect of β-nitropropionic acid onhydrolases, oxidases and transferases M. Y.H. Osman andH.M. Y. Osman. Med. Res. Inst., Alexandria, Egypt.B112 520.13 The effect of calcium on conformationalchange of thimet oligopeptidase. N.R. Pratini, B. Shaw, J.A.Sigman and M.J. Glucksman. Saint Mary’s Col. of Californiaand Rosalind Franklin Univ. of Med. and Sci.B113 520.14 Cloning and expression of putativemetacaspases from Schizophyllum commune. M. Richter.Union Col., NY.B114 520.15 Expression of a putative metacaspase genefrom Schizophyllum commune. Z. Zheng and K. Fox. UnionCol., NY.SUN143