BIOCHEMISTRY<strong>SUNDAY</strong>535. SYSTEMS BIOLOGYPosterSUN. 7:30 AM—WASHINGTON CONVENTION CENTER, EXHIBITHALL ABCChemical <strong>Biology</strong> and Drug DiscoveryStructure, Mechanism and Regulation in EnzymeCatalysisPresentation time: 1:05 PM-2:35 PMB197 535.1 Bacterial stress response to hydrogen peroxide.K. Cade. Rutgers Univ., Camden.B198 535.2 Transcriptomic and histopathologiccharacterization of mouse model(s) simulating features ofpost-traumatic stress disorder. S. Muhie, R. Hammamieh, S-A.Miller, J. Meyerhoff, E. Carroll and M. Jett. Walter Reed ArmyInst. of Res. and Naval Med. Res. Ctr.B199 535.3 Comparative developmental and evolutionarystudies of new Caenorhabditis species in Virginia. B.A. Wang,E.A. Haynes and T.M. Grana. Univ. of Mary Washington.B200 535.4 Phosphoproteome profi ling of HEK 293 cellsunder osmotic stress using SILAC. R. Wang, J.D. Ferraris, G.Wang, M. Gucek and M.B. Burg. NHLBI, NIH.536. EXPERIMENTAL AND COMPUTATIONALAPPROACHES TO SYSTEMSPosterSUN. 7:30 AM—WASHINGTON CONVENTION CENTER, EXHIBITHALL ABCPresentation time: 1:05 PM-2:35 PMB201 536.1 Minimizing model output uncertainty using aglobal, parallel model-based design of experiments approachJ.N. Bazil, G.T. Buzzard and A.E. Rundell. Med. Col. ofWisconsin and Purdue Univ. Weldon Sch. of Biomed. Engin.B202 536.2 Combining computational and experimentalapproaches to investigate the catalytic mechanism ofglyoxysomal malate dehydrogenase. H. Guterres, S. Hedrickand E. Bell. Univ. of Richmond.B203 536.3 Systematic computational and experimentalidentifi cation of trans-factors that modulate posttranscriptionalgene expression of the hypoxia inducible factor 1 alpha. R.Mrowka, A. Bondke Persson, A. Steege, A. Patzak, P.B.Persson and M. Fähling. Friedrich Schiller Univ. Jena andCharité, Med. Univ. Berlin.B204 536.4 NIGMS PSI:<strong>Biology</strong> Initiative – enabling highthroughputstructural biology and structural genomics. W.Smith, A. Ainsztein, R. Basavappa, J. Chin, C. Edmonds, P.Flicker, P. Preusch, J. Wehrle, C. Lewis and J. Berg. NIGMS,NIH.537. GENOMICS AND TOXICOGENOMICSPosterSUN. 7:30 AM—WASHINGTON CONVENTION CENTER, EXHIBITHALL ABCPresentation time: 1:05 PM-2:35 PMB205 537.1 The effect of atrazine on the thyroid axis inzebrafi sh. J.L. Sine, G.T. Ayers and D.M. Baker. Univ. of MaryWashington.538. MATHEMATICAL BIOLOGYPosterSUN. 7:30 AM—WASHINGTON CONVENTION CENTER, EXHIBITHALL ABCPresentation time: 1:05 PM-2:35 PMB206 538.1 A matrix model of fasting metabolism innorthern elephant seal pups. E.O. Keith. Nova SoutheasternUniv., FL.539. MICROARRAYSPosterSUN. 7:30 AM—WASHINGTON CONVENTION CENTER, EXHIBITHALL ABCPresentation time: 1:05 PM-2:35 PMB207 539.1 Lysophosphatidic acid regulates a complexarray of genes in human gingival fi broblasts. D.R. Cerutis, M.D.Weston, A. Ogunleye, T.P. McVaney, K.V. Headen and L.C.Parrish. Creighton Univ.B208 539.2 E3 and DUB substrate identifi cation, andchain linkage profi ling using microarrays C.M. Loch and J.E.Strickler. LifeSensors Inc., Malvern, PA.540. METHODSPosterSUN. 7:30 AM—WASHINGTON CONVENTION CENTER, EXHIBITHALL ABCPresentation time: 1:05 PM-2:35 PMB209 540.1 A one-solution, one-tube plasmid DNA miniprepL. Brunelli, M.R. Kuehn and G. Lezin. Univ. of Utah and NCI-Frederick.B2<strong>10</strong> 540.2 Hofmeister series salts enhance purifi cation ofplasmid DNA by non-ionic detergents. L. Brunelli, M.R. Kuehnand G. Lezin. Univ. of Utah and NCI-Frederick.B211 540.3 Identifi cation of Akt-interacting proteinsusing on-beads cross-linking, co-immunoprecipitation andmass spectrometry B.X. Huang and H-Y. Kim. NIAAA, NIH,Rockville.B212 540.4 Universal phosphatase-coupled glycosyltransferaseassay. W. Jiang, C. Ethen, B. Prather, M. Machacekand Z. Wu. R&D Systems Inc., Minneapolis.B213 540.5 Detection of specifi c glycosaminoglycansand glycan epitopes by in vitro sulfation using recombinantsulfotransferases. W. Jiang, B. Prather, C. Ethen, A. Kalyuzhnyand Z. Wu. R&D Systems Inc., Minneapolis.148
<strong>SUNDAY</strong>B214 540.6 A hight throughput protocol for using soilmolecular biology as trace evidence. S.A. Larson, N. Petal,J. Hustedt, R.A. Drijber, D.O. Carter and C.P. Bailey. Univ. ofNebraska-Lincoln.B215 540.7 Direct, rapid, and effi cient transesterifi cation oftotal lipids in human serum by microwave irradiation Y.H. Lin,S. Strandjord, J.D. Loewke, D.Y. Hyun, J. Leazer and J.R.Hibbeln. NIAAA, NIH and CEM Corp., Matthews, NC.B216 540.8 Characterization of a real-time PCR assayfor the detection and quantitation of Plasmodium malariaeparasites. E. Parsons, J. Koros, C. Obuya, D. Aching andA. Stewart. Kalamazoo Col., U.S. Army Med. Res.-Kenya andWalter Reed Army Inst. of Res.B217 540.9 Synthesis of 1, 13-dichloro 5, 6, 8, 9-tetraazadibenzo anthracene, helical considerations Y.A. Puplampu-Dove and G. Mandouma. Univ. of Maryland Eastern Shoreand Furman Univ.B218 540.<strong>10</strong> Chromatin immunoprecipitation: revisiting theeffi cacy of sample preparation and sonication, and of shearedDNA extraction, quantifi cation, and analysis via polymerasechain reaction P.D. S. Bortz and B.R. Wamhoff. Univ. ofVirginia.B219 540.11 The multiple solvent crystal structures ofP22TSP. M.D. Smith, C. Mattos and S. Barbiz. North CarolinaState Univ. and Univ. Potsdam, Germany.B220 540.12 Increase of BCR-ABL/ABL ratio thatcorresponds to BCR-ABL mutation detection in chronic myeloidleukemia patients treated by imatinib. G. Tsaur, A. Popov, A.Ivanova, Y. Yakovleva, T. Riger, O. Plekhanova, Y. Ivanets, E.Shorikov, L. Saveliev and L. Fechina. Reg. Children’s Hosp.#1 and Novartis Pharma, Yekaterinburg, Russia.B221 540.13 Development of a multiplex immunoassay forphosphothreonine protein analysis. R. Wiese, S. Harbison andD. Hayes. EMD Millipore, St. Charles, MO.541. MASS SPECTROSCOPYPosterSUN. 7:30 AM—WASHINGTON CONVENTION CENTER, EXHIBITHALL ABCPresentation time: 1:05 PM-2:35 PMB222 541.1 Quantifi cation of eicosanoid pathway proteinsin human cerebrospinal fl uid using a dual pressure linear iontrap mass spectrometer. R.G. Biringer, J.A. Horner, A.N.Fonteh, S. Kauffman, A.F. R. Hühmer and M.G. Harrington.Thermo Fisher Scientifi c, San Jose and Huntington Med. Res.Insts., Pasadena.B223 541.2 Characterization of specifi c lysophosphatidicacid subspecies produced by autotaxin using a novel LC/MS/MS method. E. Mayton, D.S. Wijesinghe, J. Mietla, X. Fangand C.E. Chalfant. Virginia Commonweatlh Univ. and HunterHolmes McGuire VA Med. Ctr.B224 541.3 An approach to standardize Lepidium meyenii(Maca) extracts by evaluating macamide content with an LC-MS/MS method. A.J. Pino-Figueroa, M. Bohlke, H. Wu, C.Kelley, D.A. Williams and T.J. Maher. Massachusetts Col. ofPharm. and Hlth. Sci.B225 541.4 Optimized MS quantitation of knockdown,transcription factor profi les and isoform modulation during stemcell differentiation. J. Swift, T. Harada, J-W. Shin, M. Tewari,H-Y. Tang, D.W. Speicher and D.E. Discher. The Wistar Inst.and Univ. of Pennsylvania.542. CELL CYCLEPosterSUN. 7:30 AM—WASHINGTON CONVENTION CENTER, EXHIBITHALL ABCPresentation time: 1:05 PM-2:35 PMB226 542.1 Biochemical and immunocytochemical analysisof proteins transiently associated with the chromosomalpassenger complex during mitosis. S.L. Faitar, J. Vokes, K.Pundt and Z. Wintrob. D’Youville Col., NY.B227 542.2 Nucleoporin Nup88 orchestrates properchromosomal segregation through its interaction with Cdc20during mitosis. C. Hashizume. Kanazawa Univ., Japan.543. CELL CYCLE AND GROWTH CONTROLPosterSUN. 7:30 AM—WASHINGTON CONVENTION CENTER, EXHIBITHALL ABCPresentation time: 1:05 PM-2:35 PMBIOCHEMISTRYB228 543.1 Overexpression of HDAC1 modulates cervicalcancer cells proliferation by raised expression of PP2Ac. J-Y.Chuang, W-C. Chang and J-J. Hung. Natl. Cheng Kung Univ.,Taiwan.B229 543.2 Serine-threonine kinase receptor-associatedprotein activates B-myb function through direct interaction. H.Ha, H-A. Seong and R. Manoharan. Chungbuk Natl. Univ.,South Korea.B230 543.3 The activation of P2Y11 inhibits cell proliferationby induction of cell cycle arrest and cell autophagy in endothelialcells. J. Hu, Z. Xiao, M. Yang, Q. Lv, M. Deng, X. Liu, X. Chen,M. Chen, L. Fang and X. Xie. Renmin Hosp., Wuhan Univ.and Xiangya Hosp., Central South Univ., People’s Republic ofChina.B231 543.4 Natural oils as cancer suppressors. S.J.Majeno, C.A. Ibarra and N.S. Parmar. California State Univ.Channel Islands.B232 543.5 Interferon-induced growth suppression: aGRIM path. S.C. Nallar, S. Kalakonda and D.V. Kalvakolanu.Univ. of Maryland Sch. of Med.B233 543.6 Reduction of CTGF/CCN2 induces cell cyclearrest in primary human adult skin fi broblasts. C.M. Ruth, G.Fisher, J. Chrivia, J. Baldassare, T. Quan and T. He. SaintLouis Univ. and Univ. of Michigan Med. Sch.B234 543.7 The role of hydrogen peroxide as anendogenous growth factor in human breast cancer cells. S.Sen, B. Kawahara and G. Chaudhuri. UCLA.B235 543.8 Molecular mechanisms by which ciclopiroxinhibits proliferation and induces apoptosis of tumor cells. T.Shen, H. Zhou and S. Huang. LSU Hlth. Sci. Ctr., Shreveport.SUN149