SUNDAY, APRIL 10 Across Societies – Experimental Biology

SUNDAYD375 655.7 Mechanism of high NaCl- and urea-inducedinhibition of GDPD5, an enzyme whose phosphodiesteraseactivity breaks down the osmoprotective organic osmolyteglycerophosphocholine S. Topanurak, J.D. Ferraris, J. Li, C.K.Williams and M.B. Burg. NHLBI, NIH.D376 655.8 Rac1/OSM and PLC-gamma1 contributein combination to high NaCl-induced activation of theosmoprotective transcription factor TonEBP/OREBP. X. Zhou,M.B. Burg and J.D. Ferraris. Uniformed Svcs. Univ. of Hlth.Sci. and NHLBI, NIH.D377 655.9 Evidence for participation of aquaporins duringcell volume regulation in nematocytes from acontia of Aiptasiamutabilis (Cnidaria, anthozoa) A. Marino, R. Morabito, G. LaSpada, N.C. Adragna and P.K. Lauf. Univ. of Messina, Italyand Wright State Univ.D378 655.10 NFAT5 chromatin immunoprecipitation assayusing NFAT5 knockdown cells as control. Y. Izumi, N.I.Dmitrieva, J. Ferraris and M.B. Burg. NHLBI, NIH.656. INTRACELLULAR PH AND ACID-BASETRANSPORTPosterSUN. 7:30 AM—WASHINGTON CONVENTION CENTER, EXHIBITHALL ABCPresentation time: 12:45 PM-3:00 PMD379 656.1 Roles of NHE1 and NBCn1 in pH-regulationand chemotherapy resistance in MCF-7 breast cancer cellsexpressing constitutively active ErbB2. G. Lauritzen, R.Dybboe, E. Boedtkjer, C. Aalkjaer, J. Nylandsted and S.F.Pedersen. Univ. of Copenhagen, Univ. of Aarhus and DanishCancer Soc., Copenhagen.D380 656.2 Novel roles of NHE1 in motility of serumstarvedMCF7 breast cancer cells expressing constitutivelyactive ErbB2. S.F. Pedersen, G. Lauritzen, A. Gorbatenko,S.F. Lund, R. Dybboe, A. Schwab and C-M. Stock. Univ. ofCopenhagen and Univ. of Muenster.D381 656.3 Unusual transport modes of the Na + -coupledHCO 3-transporters NDCBE (SLC4A8) and NBCN2 (SLC4A10)in the absence of extracellular Cl - . M.D. Parker and W.F. Boron.Case Western Reserve Univ. Sch. of Med.D382 656.4 Role of Na-coupled HCO3 transporters inregulation of intracellular pH in rat hippocampal neuronsunder normoxic and chronic hypoxic conditions. V.A. Ruffin,P. Bouyer and W.F. Boron. Case Western Reserve Univ. andUniv. of Chicago.D383 656.5 Structural characterization of the kidney-specifi cform of the electrogenic sodium bicarbonate cotransporterNBCe1-A. N. Abuladze, I. Atanasov, K. Tsirulnikov, J. Jiang,N. Magilnick, D. Newman, Z. Zhou, A. Pushkin and I. Kurtz.UCLA.D384 656.6 Role of Ca 2+ in phosphatidylinositol4,5-bisphosphate (PIP 2)/IP 3-induced stimulation of theelectrogenic Na/bicarbonate cotransporter NBCe1-Cheterologously expressed in Xenopus laevis oocytesI.M. Thornell and M.O. Bevensee. Univ. of Alabama atBirmingham.D385 656.7 The role of carbonic anhydrase II on HCO 3--initiated transport through the SLC4A4 transporter NBCe1. F.J.Moss and W.F. Boron. Case Western Reserve Univ.D386 656.8 NBCe1 (SLC4A4) functions as a dimer andN-termini are required for unifying the transporter. M-H. Chang,H.L. Holmes and M.F. Romero. Mayo Clin. Col. of Med.D387 656.9 Distinguishing hco 3-from co 32-transport bynbce1-a. S-K. Lee, I.I. Grichtchenko and W.F. Boron. CaseWestern Reserve Univ. and Univ. of Colorado Denver, Aurora.D388 656.10 Transport stoichiometry of the electrogenicsodium bicarbonate cotransporter NBCe2 estimated with anovel delta current method. X.M. Shao, L. Kao and I. Kurtz.UCLA.D389 656.11 Regulation of acidosis-induced V-ATPasetraffi cking in salivary ducts by protein phosphorylation. O.Oehlke, C. Schlosshardt and E. Roussa. Univ. of Freiburg,Germany.657. TRANSPORTERS: TRANSMITTERS,NUTRIENTS, METABOLITES AND DRUGSPosterSUN. 7:30 AM—WASHINGTON CONVENTION CENTER, EXHIBITHALL ABCPresentation time: 12:45 PM-3:00 PMPHYSIOLOGYD390 657.1 PDZ-containing protein PDZD11 is associatedwith the human sodium-dependent multivitamin transporter,hSMVT in intestinal epithelial cells S.M. Nabokina, V.S.Subramanian and H.M. Said. Univ. of California, Irvine VAMed. Prog., Long Beach.D391 657.2 Roles of the putative N-glycosylation (Asn138and asn489) and PKC-phosphorylation (Ser283 and Thr286)sites of the human sodium-dependent multivitamin transporterin functionality and regulation. A. Ghosal, V.S. Subramanianand H.M. Said. Univ. of California, Irvine and VA Med. Ctr.,Long Beach.D392 657.3 Structure-function activity of the humansodium-dependent multivitamin transporter: role of histidineresidues. A. Ghosal and H.M. Said. Univ. of California, Irvine/VA Med. Ctr., Long Beach.D393 657.4 Does the HK2 cell line represent a suitablemodel for the study of drug transporter expression by renalepithelial cells? S.E. Jenkinson, E. van Loon, A. Dalzell andC. Brown. Newcastle Univ., U.K.D394 657.5 Effect of chronic alcohol consumption on biotintransport in rat kidneys. V. Subramanian, S.B. Subramanyaand H.M. Said. Univ. of California, Irvine and VA Med. Ctr.,Long Beach.D395 657.6 Characterization of serotonin transporterfunction in immortalized human lymphoblastoid cell lines. N.Hill-Kapturczak, D.M. Dougherty, D. Galindo and M. Javors.Univ. of Texas Hlth. Sci. Ctr. at San Antonio.D396 657.7 Screen of human Na+/dicarboxylatecotransporter 1 interacting proteins using the split-ubiquitinmembrane yeast two-hybrid method. J. Chung and A.M. Pajor.Skaggs Sch. of Pharm. and Pharmaceut. Sci., UCSD.D397 657.8 KCC2a expression in a primary fetal humallens epithelial cell line. V. Srivastava, P.K. Lauf, M. Di Fulvio,N.C. Adragna and N. Sharma. Wright State Univ.D398 657.9 The role of N-glycosylation in the intestinal Cl - /HCO 3-exchanger SLC26A3. H. Hayashi, Y. Yamashita and Y.Suzuki. Univ. of Shizuoka, Japan.D399 657.10 Verapamil and dantrolene inhibit creatinetransport in skeletal and cardiac myocytes. J.G. Mantilla, D.O.Jacobs and L. Santacruz. Duke Univ. Med. Ctr.SUN199