NanoLC 1D Plus and 2D System Operator's Manual - Eksigent

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8 Operator’s Manualstep 5step 6step 7step 8Place the instrument on a lab bench.Check the contents of the accessory kit against the contents checklist in Appendix A to confirm thatall items are included.The checklist for parts included with the nanoLC Autosampler is included in the nanoLCAutosampler manual.Unpack the computer and monitor and verify that no parts are missing or damaged.2.3 Placement of the SystemPlace the nanoLC system on a lab bench in a location with convenient access to power and a source of100 psi (6.9 bar) regulated clean, dry air or nitrogen. The front of the instrument should be accessibleat all times. The sides and back of the instrument should be clear to accommodate mobile phasereservoir placement and attachment, and computer cable attachments2.4 Connecting to the Air SupplyOperation of the instrument requires connection to a source of 100 psi (6.9 bar) regulated clean, dry airor nitrogen as described in the section on Site Requirements.Figure 2-1. Rear Panel - Air ConnectionRequired tools and materials:• Clean, dry source of air or nitrogen at 100 psi (6.9 bar)• In-line moisture trap with ¼” end-fittings• 6 mm or ¼” air supply line (included)• Quick-connect adaptor to 6 mm or ¼” supply line (included)step 1step 2step 3Connect the supplied air line to a source of 100 psi (6.9 bar) regulated clean, dry air or nitrogenusing the supplied quick-connect adaptor. Connect the other end of the air line to the air connectionon the back of the nanoLC system (see Figure 2-1).Turn on the air source to the nanoLC system.Test for air leaks. Turn off air supply and repair any air leaks that are found.nanoLC Operator’s Manual 611-00012 Revision A1
System Installation 92.5 Connecting the PCRequired materials:• Computer, keyboard and mouse• nanoLC system serial cable (included)Figure 2-2. Rear Panel — PC I/O ConnectorConnect one end of the RS-232 cable to an available COM port connector on the computer. COM1 isrecommended as it is selected as the default in the software configuration.The other end of the cable should be connected to the 9-pin connector labeled PCI/O on the rearpanel of the nanoLC system (see Figure 2-2). Tighten the retaining screws at both ends to secure thecable in place.2.6 Connecting the nanoLC to PowerRequired materials:• 24 VDC power supply (included)Insert the supplied 24 VDC power supply’s plug into the connector located on the back of theinstrument (Figure 2-3). Plug the line voltage cord into an appropriately grounded line voltage outlet.Turn the nanoLC on using power switch on the back of the unit.24 VAC PowerFigure 2-3. Rear Panel — Power ConnectionnanoLC Operator’s Manual 611-00012 Revision A1
Page 1 and 2: operator’s manualplus systemsyste
Page 3 and 4: iiiTable of ContentsChapter 1. Safe
Page 5 and 6: Chapter 1.Safety and Site Requireme
Page 7 and 8: Safety and Site Requirements 3Do no
Page 9: 1.4.4 Bench Space RequirementSafety
Page 14 and 15: 10 Operator’s Manual2.7 Installin
Page 16 and 17: 12 Operator’s ManualFigure 2-8. I
Page 18 and 19: 14 Operator’s ManualFigure 2-11.
Page 20 and 21: 16 Operator’s ManualRequired tool
Page 22 and 23: 18 Operator’s ManualHold down the
Page 25 and 26: System Initialization 21step 5step
Page 28 and 29: 24 Operator’s Manual4.2 Disposing
Page 30 and 31: 26 Operator’s ManualFigure 4-1. D
Page 32 and 33: 28 Operator’s ManualFigure 4-4. A
Page 34 and 35: 30 Operator’s ManualFigure 4-8. F
Page 37 and 38: Chapter 5.Quick-start GuideChapter
Page 39 and 40: Quick-start Guide..35CloseLoosenGri
Page 41 and 42: 5.4 Creating an Autosampler MethodQ
Page 43 and 44: Quick-start Guide..39Table 5.1. Tem
Page 45 and 46: step 2 To create a new method, type
Page 47 and 48: Quick-start Guide..43step 11 The mo
Page 49 and 50: Quick-start Guide..45step 10 Use th
Page 51: Quick-start Guide..47Figure 5-17. A
Page 54 and 55: 50 Operator’s Manual6.2 Calibrati
Page 56 and 57: 52 Operator’s Manual6.4 Troublesh
Page 58 and 59: 54 Operator’s ManualTable 6-1. (c
Page 60 and 61: 56 Operator’s ManualAutosampler
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58 Operator’s Manual6.5 Error Mes
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60 Operator’s ManualA.2 Replaceme
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Appendix BExternal InterfaceB.1 Int
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External Interface 65B.2 Valve Conn
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External Interface 67B.3.3 Triggeri
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70 Operator’s ManualFigure C-2 sh
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72 Operator’s ManualFigure C-4 sh
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74 Operator’s ManualSelect: Autos
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76 Operator’s ManualDefault metho
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78 Operator’s ManualRun in Synchr
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IndexFlow Rate Verification, 21AFlo
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