NanoLC 1D Plus and 2D System Operator's Manual - Eksigent

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NoticeSupportAt Eksigent Technologies, we are committed to providing the highest level of support to our users. To obtainanswers to questions about any of our products, report problems, or suggest improvements, please visitwww.Eksigent.com. For on-site service, support and training, please contact your local Eksigent Technologiessales representative or customer service representative or send an e-mail to support@eksigent.com.NoticeThe information in this document is subject to change without notice and should not be construed as acommitment by Eksigent Technologies. Eksigent Technologies assumes no responsibility for any errors that mayappear in this document. This document is believed to be complete and accurate at the time of publication. Inno event shall Eksigent Technologies be liable for incidental or consequential damages in connection with orarising from the use of this document.iiEksigent Technologies makes no warranties or representations as to the fitness of this equipment for anyparticular purpose and assumes no responsibility or contingent liability, including indirect or consequentialdamages, for any use to which the purchaser may put the equipment described herein, or for any adversecircumstances arising therefrom.This document is provided to customers who have purchased Eksigent Technologies equipment to use in theoperation of such equipment. This document is copyright protected and any reproduction of this document orany part of this document is strictly prohibited, except as Eksigent Technologies may authorize in writing.Equipment described in this document is protected under one or more patents filed in the United Statesand other countries. Additional patents are pending.Software described in this document is furnished under a license agreement. It is against the law to copy,modify, or distribute the software on any medium, except as specifically allowed in the license agreement.Furthermore, the license agreement may prohibit the software from being disassembled, reverse engineered, ordecompiled for any purpose.Portions of this document may make reference to other manufacturers ' products, which may contain parts thatare patented and may contain parts whose names are registered as trademarks and/or function as trademarks.Any such usage is intended only to designate and describe those manufacturer ' s products and does not implyany right and/or license use or permit others to use such product names as trademarks. All products andcompany names mentioned herein may be the trademarks of their respective owners.Eksigent ® is a registered trademark owned by Eksigent Technologies in the United States and certain othercountries.For Research Use Only. Not for use in diagnostic procedures.Eksigent Technologies5875 Arnold Rd.Dublin, CA 94568tel: 925-560-2600 fax: 925-560-2700web: www.eksigent.comCopyright © 2007 Eksigent Technologies LLC. All rights reserved.Printed in the United States of AmericananoLC Operator’s Manual 611-00012 Revision A1
iiiTable of ContentsChapter 1. Safety and Site Requirements .....................................11.1 Safety Conventions ....................................................................................................11.2 nanoLC Safety Practices ..........................................................................................11.3 nanoLC Autosampler Safety Practices..................................................................21.4 Site Requirements ......................................................................................................41.4.1 nanoLC Power Requirements ....................................................................41.4.2 nanoLC Autosampler Power Requirements ...........................................41.4.3 Air Supply Requirements.............................................................................41.4.4 Bench Space Requirement.........................................................................51.4.5 Environment Requirements.........................................................................5Chapter 2. System Installation ........................................................72.1 nanoLC System Overview ........................................................................................72.2 Unpacking the nanoLC System...............................................................................72.3 Placement of the System ..........................................................................................82.4 Connecting to the Air Supply...................................................................................82.5 Connecting the PC.....................................................................................................92.6 Connecting the nanoLC to Power ..........................................................................92.7 Installing Software and Instrument Settings...................................................... 102.8 Configuring the nanoLC System.......................................................................... 112.9 Configuring the nanoLC Autosampler ................................................................ 13Chapter 3. System Initialization....................................................153.1 Hardware Components and Functions............................................................... 153.2 Loading Mobile Phases .......................................................................................... 153.3 Preparing the nanoLC Autosampler.................................................................... 193.4 Flushing the Autosampler Syringe and Liquid Path......................................... 203.5 Connecting the nanoLC to the nanoLC Autosampler..................................... 203.6 Verifying the Flow Rate........................................................................................... 21Chapter 4. Routine Maintenance ..................................................234.1 Recommended Maintenance ................................................................................ 234.2 Disposing of Waste................................................................................................. 244.3 Changing the Sample Loop................................................................................... 244.4 Replacing Capillary Connections......................................................................... 254.5 Zeroing the Pressure Transducers ...................................................................... 264.6 Checking Flow Stability.......................................................................................... 284.7 Autotuning Flow Controllers.................................................................................. 284.8 Calibrating the Flow Meters .................................................................................. 294.9 Cleaning and Inspecting the Instrument............................................................. 31nanoLC Operator’s Manual 611-00012 Revision A1
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Appendix BExternal InterfaceB.1 Int
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External Interface 65B.2 Valve Conn
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IndexFlow Rate Verification, 21AFlo
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NanoLC 1D Plus and 2D System Operator's Manual - Eksigent

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