NanoLC 1D Plus and 2D System Operator's Manual - Eksigent

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66 Operator’s ManualB.3.1 Other AutosamplersThe nanoLC can be integrated with autosamplers from other vendors through the use of contactclosures. A variety of configurations are possible using the inputs and outputs of the nanoLC describedabove. One suggested configuration is described here.Setup up the injection method and sample sequence using the autosampler interface (front panel orother software). Setup a matching sequence of chromatographic methods in the Run Manager of thenanoLC. Also make sure that LC Wait for Contact Closure is checked under the System menu in theRun Manager Window. Make sure that the analysis time per sample (time between autosamplerinjections) in the autosampler method is long enough to allow for the nanoLC method run time plus anypre-run flush equilibration for the next run.Connect the inject marker out from the autosampler to the Run Input (pin 8) and Ground (pin 7) of thenanoLC. Start the sequence by starting the Run Manager sequence and then the autosampler. After theautosampler initiates its own sequence for dispensing sample it will trigger the nanoLC to start itschromatographic run.The nanoLC also provides a Ready Out (pin 5) which can be used as an input to other autosamplers tosynchronize runs and error check operation.Note: The optimized injection routines that can be utilized with the nanoLC Autosampler will, ingeneral, not be available with other autosamplers. While the rapid inject or metered injection routinesof the nanoLC method can be used to increase the flow rate during sample loading, one will not beable to switch the sample loop out of the flow path during the gradient. The may lead to significantlyincreased gradient delay times and decreased response times of the system.B.3.2 Triggering MS Data CollectionWhile integrated control of the nanoLC system through the Xcalibur and Analyst software packages willbe available, most MS systems will allow synchronization of data collection through the use of contactclosures. The following describes the general configuration for this synchronization.In the MS software package, choose the option to start data collection with an external contact closure.This can be applied for a single MS run or to a sequence or batch of data collection methods. Whenconducting multiple runs in a sequence table, please configure the Run Manager of the nanoLCsoftware with the corresponding sequence of methods.Set the Run Output configuration to contact closure (box checked) in the nanoLC soft-ware (menu).One must then identify the hardware interface for the MS contact closure input. This is typically either aterminal block mounted on the side of the MS instrument, or a pair of wires in an interface cablesupplied with the MS.To synchronize MS data with the beginning of the gradient (after the injection), connect the Run Out(pin 3) and the Ground terminals to the MS contact closure. If one lead of the MS trigger is ground,make sure to connect it to the Ground connection of the nanoLC. For most applications, thisconnection will be made to the channel 2 (low flow gradient) side of the instrument.One can also choose to synchronize MS data with the beginning of the injection. To do this, connectthe Valve Out (pin 6) and the Ground terminals to the MS contact closure.nanoLC Operator’s Manual 611-00012 Revision A1
External Interface 67B.3.3 Triggering Peak ParkingPeak parking on the nanoLC system can also be triggered externally using the back panel I/Oconnectors. Connect the ground of the triggering contact closure to a Ground terminal on the backpanel and connect the second wire of the contact closure to the Park In terminal (pin 9). The Park Outterminal (pin 4) can also be used to monitor the state of Peak Parking. The Peak Parking Toolbox inthe nanoLC software is used to configure the Peak Parking flow rate, flow rate reference (typically‘column’), and provide for hold and lockout times on triggering.Peak Parking can be configured on ThermoFinnigan MS systems that provide a configurable con-tactclosure output. This variable contact closure can be configured in the Xcalibur software to be activatedbased on selected criteria including ion intensities and ion inclusion lists. A number of systems may alsoallow a fixed time or duration contact closure that can be configured in the MS software to trigger peakparking. These may be useful for well defined experiments that will benefit from peak parking at a knowntime (not MS-dependent signals).B.3.4 Ready OutWhen using the nanoLC Autosampler, one can also handshake with other components, such as a MSdetector, using the Ready Out from that device. This will allow the LC queue to stop if the peripheral isnot ready. To do this, connect the Ready Out from the peripheral to the In 3 and Gnd of the AS1 Input(P6) cable. Add the following line to the beginning of the AS1 method.‘Wait for Input 3-LOW’Note: If the Ready Out from the peripheral is a TTL high trigger instead of TTL low or contact closure,change the line to 3-HIGH.The AS method will not proceed beyond the first line until it has received a ready signal. If youdisconnect the peripheral, be sure to remove this line from the AS method or to short In 3 to ground.nanoLC Operator’s Manual 611-00012 Revision A1
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Page 89 and 90: IndexFlow Rate Verification, 21AFlo
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NanoLC 1D Plus and 2D System Operator's Manual - Eksigent

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