NanoLC 1D Plus and 2D System Operator's Manual - Eksigent

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52 Operator’s Manual6.4 Troubleshooting ChecklistSystem Initialization…Table 6-1. Troubleshooting ChecklistSymptom Possible Cause Corrective ActionPower LED on front panel is noton.Power cord not connectedNo power at outletPower LED has failed but systemresponse OKConnect power cord.Repair electrical outlet.Contact Eksigent TechnologiesTechnical Support for assistance.Front panel power LED is on butsoftware fails to recognize theinstrument’s presence.Communication error betweencomputer and an LC systemVerify that the instrument’s serial cableis securely connected to the computer’sCOM1 serial port. (see Section2.5) Reboot computer and cycle poweron instrument.Contact Eksigent TechnologiesTechnical Support for assistance.Liquid leaking at the bottom of themobile phase reservoirsThe reservoirs were not placed inthe pump adapter properlyVerify the stopcock is placed properlyand tightly screwed in.Loud hissing sound from theinstrumentAir leaks from the air inlet fittingVerify the air tubing is connectedproperly to the gas fitting.Tighten the air inlet gas fitting.Contact Eksigent TechnologiesTechnical Support for assistance.nanoLC Operator’s Manual 611-00012 Revision A1
Quick-start Guide..53Flow Control System…Table 6-1. (cont.) Troubleshooting ChecklistSymptom Possible Cause Corrective ActionSystem pressure (Pc) and/orpump pressures (Pa & Pb) showpressure even though the flow isoffPressure transducers zero offsetsare incorrectZero pressure transducers (Section4.5)No liquid out of waste line whenpurgingAir trap in the pumpInternal filters are pluggedLeak in the system prior to thepurge valvePrime the pump (Section 3.2).Contact Eksigent TechnologiesTechnical Support for assistance.Check all connections.Pump restrokes frequently- Pumphas reached end of stroke errormessage occursPump does not restroke at the endof a runAir trapped in the pumpPump remains on long enough toprompt a re-strokeCheck valve is leakingPump restroke delay is too shortPrime the pump (Section 3.2).Check the duration of time between restrokesto see if the pump re-strokewas appropriate.Contact Eksigent TechnologiesTechnical support for assistance.Contact Eksigent TechnologiesTechnical Support for assistance.Pump flushes out quickly but doesnot deliver ~600 µL per stroke.Leak in instrumentContact Eksigent TechnologiesTechnical Support for assistance.Purge output drips out slowly Internal filters are plugged Contact Eksigent TechnologiesTechnical Support for assistance.No flow rate with 100% powerindicated. System pressure (Pc)and pump pressures (Pa & Pb)are all lowNo air to systemSystem not properly primed andflushedConnect 100 psi clean, dry air to theinstrument’s air inlet.Prime and flush system (Section 3.2).Inability to reach desired flow rateInternal filters are pluggedFlow rate set point too high forsystem back pressureAir pressure too lowContact local Eksigent TechnologiesTechnical Support to replace internalfilters.Reset flow rate to a lower level.Establish correct air pressure (100 psi).nanoLC Operator’s Manual 611-00012 Revision A1
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operator’s manualplus systemsyste
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iiiTable of ContentsChapter 1. Safe
Page 5 and 6: Chapter 1.Safety and Site Requireme
Page 7 and 8: Safety and Site Requirements 3Do no
Page 9: 1.4.4 Bench Space RequirementSafety
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Page 14 and 15: 10 Operator’s Manual2.7 Installin
Page 16 and 17: 12 Operator’s ManualFigure 2-8. I
Page 18 and 19: 14 Operator’s ManualFigure 2-11.
Page 20 and 21: 16 Operator’s ManualRequired tool
Page 22 and 23: 18 Operator’s ManualHold down the
Page 25 and 26: System Initialization 21step 5step
Page 28 and 29: 24 Operator’s Manual4.2 Disposing
Page 30 and 31: 26 Operator’s ManualFigure 4-1. D
Page 32 and 33: 28 Operator’s ManualFigure 4-4. A
Page 34 and 35: 30 Operator’s ManualFigure 4-8. F
Page 37 and 38: Chapter 5.Quick-start GuideChapter
Page 39 and 40: Quick-start Guide..35CloseLoosenGri
Page 41 and 42: 5.4 Creating an Autosampler MethodQ
Page 43 and 44: Quick-start Guide..39Table 5.1. Tem
Page 45 and 46: step 2 To create a new method, type
Page 47 and 48: Quick-start Guide..43step 11 The mo
Page 49 and 50: Quick-start Guide..45step 10 Use th
Page 51: Quick-start Guide..47Figure 5-17. A
Page 54 and 55: 50 Operator’s Manual6.2 Calibrati
Page 58 and 59: 54 Operator’s ManualTable 6-1. (c
Page 60 and 61: 56 Operator’s ManualAutosampler
Page 62 and 63: 58 Operator’s Manual6.5 Error Mes
Page 64 and 65: 60 Operator’s ManualA.2 Replaceme
Page 67 and 68: Appendix BExternal InterfaceB.1 Int
Page 69 and 70: External Interface 65B.2 Valve Conn
Page 71: External Interface 67B.3.3 Triggeri
Page 74 and 75: 70 Operator’s ManualFigure C-2 sh
Page 76 and 77: 72 Operator’s ManualFigure C-4 sh
Page 78 and 79: 74 Operator’s ManualSelect: Autos
Page 80 and 81: 76 Operator’s ManualDefault metho
Page 82 and 83: 78 Operator’s ManualRun in Synchr
Page 89 and 90: IndexFlow Rate Verification, 21AFlo
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