NanoLC 1D Plus and 2D System Operator's Manual - Eksigent

Quick-start Guide..53Flow Control System…Table 6-1. (cont.) Troubleshooting ChecklistSymptom Possible Cause Corrective ActionSystem pressure (Pc) and/orpump pressures (Pa & Pb) showpressure even though the flow isoffPressure transducers zero offsetsare incorrectZero pressure transducers (Section4.5)No liquid out of waste line whenpurgingAir trap in the pumpInternal filters are pluggedLeak in the system prior to thepurge valvePrime the pump (Section 3.2).Contact Eksigent TechnologiesTechnical Support for assistance.Check all connections.Pump restrokes frequently- Pumphas reached end of stroke errormessage occursPump does not restroke at the endof a runAir trapped in the pumpPump remains on long enough toprompt a re-strokeCheck valve is leakingPump restroke delay is too shortPrime the pump (Section 3.2).Check the duration of time between restrokesto see if the pump re-strokewas appropriate.Contact Eksigent TechnologiesTechnical support for assistance.Contact Eksigent TechnologiesTechnical Support for assistance.Pump flushes out quickly but doesnot deliver ~600 µL per stroke.Leak in instrumentContact Eksigent TechnologiesTechnical Support for assistance.Purge output drips out slowly Internal filters are plugged Contact Eksigent TechnologiesTechnical Support for assistance.No flow rate with 100% powerindicated. System pressure (Pc)and pump pressures (Pa & Pb)are all lowNo air to systemSystem not properly primed andflushedConnect 100 psi clean, dry air to theinstrument’s air inlet.Prime and flush system (Section 3.2).Inability to reach desired flow rateInternal filters are pluggedFlow rate set point too high forsystem back pressureAir pressure too lowContact local Eksigent TechnologiesTechnical Support to replace internalfilters.Reset flow rate to a lower level.Establish correct air pressure (100 psi).nanoLC Operator’s Manual 611-00012 Revision A1