NanoLC 1D Plus and 2D System Operator's Manual - Eksigent

Quick-start Guide..45step 10 Use the flow profile tab to survey the gradient you created to make sure it is correct. The mobilephase composition can also be set by clicking and dragging points on the graph or by setting the %of A or B in the Profile Editor region.step 11 Once the method is complete, click Save to save the analysis method. Click OK to close the editor.5.7 Creating the Run TableThe Run Table ties together an autosampler and one or more LC method with a sample vial and trayposition. You can also enter descriptive information related to the sample or analysis. This section willcreate a Run Table to run two samples with a trap and elute method.step 1 If not already in the Run Manager, click the Run Manager button located on the Eksigent ControlSoftware’s Acquisition Window. (Figure 5-1).step 2 Create a new, blank Run Table by selecting Edit > Erase Table (Figure 5-15).Figure 5-15. Creating a New Run Tablestep 3 Select File > Save As… and type trap loading in the File name field.step 4 Click Save.step 5 In the first line in the Run Table double click on the Autosampler Method field and select theautosampler method micropickup_1uL_trap loading from the drop down menu.step 6 Next, enter 1 for the tray and A1 for the sample Vial location. Alternatively, vial location can beentered by clicking at the vial on the picture of the vial tray in the Run Manager window.step 7 Double click on the LC Method field and select CH1 Trap Loading. Indicate Channel 1 under theChannel column.step 8 In line 2, leave the autosampler method, tray and vial location blank. Double click on the LC Methodfield and choose CH2 gradient. Indicate Channel 2 under the channel column.step 9 For each sample you wish to run, duplicate these two lines in succession. In Figure 5-16, twosample injections are indicated.nanoLC Operator’s Manual 611-00012 Revision A1