NanoLC 1D Plus and 2D System Operator's Manual - Eksigent

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50 Operator’s Manual6.2 Calibration ValuesThe Calibration Values tab of the Hardware Diagnostics Window summarizes the current K values ofthe flow meter, the gain and zero offsets of pressure transducers and the PID parameters for thepumps. The following settings are typical for a nanoLC and are shown for example only. The window onthe left is for channel 1 and the window on the right is for channel 2,Figure 6-1. Hardware Diagnostics — Calibration Values TabThe values should be documented before and after as part of maintaining a good instrument log, andnoting changes. These values should be very similar from diagnostic to diagnostic; large changes canindicate a problem.nanoLC Operator’s Manual 611-00012 Revision A1
Quick-start Guide..516.3 General Troubleshooting GuidelinesTo avoid the possibility of electric shock, never disconnect an electrical assembly while power isapplied to the system. After turning power off, wait about ten seconds before disconnecting anassembly.To prevent injury, always observe good laboratory practices when you handle solvents, changetubing, or operate the system. Know the physical and chemical properties of the solvents. Refer tothe Material Safety Data Sheets for the solvents in use.There are no user serviceable components or assemblies inside the nanoLC. Service or anyinternal parts or assemblies requires a Factory Qualified Service Technician.When troubleshooting the nanoLC system, follow these safety practices:The basic steps for nanoLC system troubleshooting are:step 1step 2Step back and look at the overall system. Is something obvious causing the problem? For example, isan instrument unplugged or improperly connected?Compare current system operation with the way the system operated before the problem started.Identify conditions (pressures, power settings, flow rates) that are different than they were when thesystem was operating normally.For example, if the output pressure is usually 500 psi with a certain method, is the system pressurecurrently in the same range, or drastically higher (possibly caused by a plug) or lower (possiblycaused by a leak)?step 3Identify in the order listed below the symptom that varies from normal system operation:• System power on and initialization (initialization fails)• System diagnostics (flow stability, controller tuning)• Flow rate in each channel (high, low, erratic)• Output pressure (high, low, erratic)step 4step 5For each isolated symptom, identify a list of possible causes using the troubleshooting checklist below.The troubleshooting in section 6.5 allows you to narrow down the possible causes of a symptomand find suggested corrective actions.If this process does not correct the problem, contact Eksigent Technologies Technical Support.nanoLC Operator’s Manual 611-00012 Revision A1
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Page 69 and 70: External Interface 65B.2 Valve Conn
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Page 89 and 90: IndexFlow Rate Verification, 21AFlo
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NanoLC 1D Plus and 2D System Operator's Manual - Eksigent

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