NanoLC 1D Plus and 2D System Operator's Manual - Eksigent

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46 Operator’s ManualFigure 5-16. Run Manager Window — Two Samplesstep 10 Check all the lines you wish to run under the Run column.step 11 Run the sequence in Sequential mode. Click Flush/Equilibrate when Idle to start all the pumps if youwish. Note: If you need to edit the first two lines, un-check flush/equilibrate to do so.step 12 Now that the run table has been defined (Figure 5-16), save it by selecting File > Save.5.8 Starting a RunSamples to be analyzed are selected by placing a check mark in the box to the right of the appropriaterow numbers in the Run Table. For this series of runs make sure there are check marks in the boxes forthe first four rows of the Test Table created in the previous section.Checking the Flush/Equilibrate when Idle will initiate the pre-run flush for the first method. With thisoptions selected, the system will continue to flush at the end of the sequence.Initiate the sequence of analyses by clicking on Start. After the start button has been pressed, it willchange to a red ‘Stop’ button that can be used to abort the run at any point during the analysis.Once the flow rate has stabilized, the sample injection process will begin. Channel 1 will run first, thenChannel 2 will start at the conclusion of the Channel 1 run.While the run is in progress, the Acquisition Window can display the specified flow profiles for solventsA and B as well as their actual flow rates (Qa and Qb). Traces can be added or deleted from thedisplay by clicking on System > Appearance in the Acquisition Window (Figure 5-1) and selecting thedesired items.To zoom in on a particular area of the chromatogram, click on the display and drag a box around thearea of interest. This will enlarge that area of the chromatogram. To zoom back out, right click andselect Zoom Out or Back.nanoLC Operator’s Manual 611-00012 Revision A1
Quick-start Guide..47Figure 5-17. Acquisition Window — Flow ProfilesStatus information such as %A, %B and Time Remaining are also displayed at the bottom of thescreen during the run. Status bars at the top of the screen display the actual flow rate for pump A (Qa)and pump B (Qb) in nL/min, and pressure, in psi, for pump A (Pa), pump B (Pb) and column (Pc).5.9 Viewing the Collected Data FilesPreviously collected data files can be re-opened, reviewed and re-processed.step 1 To view the data file collected from the first chromatogram, click on File > Open… then select thedata file. The nanoLC has an option to collect an external signal (e.g. a UV detector) through the A/Dinput on the 12-pin I/O connector. This data will be stored, along with the flow profile data, in thedata file. The Control software also includes a data analysis package and this analysis can be appliedto the collected A/D signal. See the software manual for further instructions on analyzing data fileswith the software.nanoLC Operator’s Manual 611-00012 Revision A1
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Page 3 and 4: iiiTable of ContentsChapter 1. Safe
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Page 7 and 8: Safety and Site Requirements 3Do no
Page 9: 1.4.4 Bench Space RequirementSafety
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Page 20 and 21: 16 Operator’s ManualRequired tool
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Page 25 and 26: System Initialization 21step 5step
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Page 41 and 42: 5.4 Creating an Autosampler MethodQ
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Page 45 and 46: step 2 To create a new method, type
Page 47 and 48: Quick-start Guide..43step 11 The mo
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Page 67 and 68: Appendix BExternal InterfaceB.1 Int
Page 69 and 70: External Interface 65B.2 Valve Conn
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Page 82 and 83: 78 Operator’s ManualRun in Synchr
Page 89 and 90: IndexFlow Rate Verification, 21AFlo

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