NanoLC 1D Plus and 2D System Operator's Manual - Eksigent

Routine Maintenance 254.4 Replacing Capillary ConnectionsRequired tools and materials:• Laboratory wipes• Clean HPLC-grade methanol• 1/4” Allen wrench• Transfer capillary• For the high flow channel, use 50 µm ID (p/n 910-00002)• For the low flow channel, use 25 µm ID (p/n 910-00008)• Microtight fitting• Green PEEK sleeve (p/n 910-00025)• Black microtight ferrule (p/n 920-00002)• Tan microtight nut (p/n 920-00003)• 1/16” sleeved fitting• 1/16” OD Orange PEEK sleeve (p/n 910-00024)• 1/16” PEEK fitting nut (p/n 920-00006)• 1/16” PEEK fitting ferrule (p/n 910-00023)step 1step 2step 3step 4step 5step 6step 7step 8Use the 1/4” Allen wrench or your fingers to remove the current fitting, ferrule, and sleeve.Remove the fitting and ferrule from the capillary and discard the old capillary.Cut the capillary to the appropriate length. Ensure a clean end cut and clean with methanol.Insert one end of a new capillary through the appropriate PEEK sleeve.Slide the end of the capillary and the PEEK sleeve through the compression fitting and ferrule. Makesure the sleeve extends through the ferrule.Slide the capillary, sleeve and fitting into the port until both bottom out.While lightly pressing the capillary and sleeve into the fitting with one hand, use the other hand or the1/4” wrench to tighten the fitting.Open the Direct Control window by clicking System > Direct Control from the Control software’sAcquisition Window (Figure 4-1).nanoLC Operator’s Manual 611-00012 Revision A1