NanoLC 1D Plus and 2D System Operator's Manual - Eksigent

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16 Operator’s ManualRequired tools and materials:• Mobile phase reservoirs (p/n 800-00105)• Clean, degassed HPLC-grade mobile phase A (water with 2% acetonitrile and 0.1% formic acidsuggested).• Clean, degassed HPLC-grade mobile phase B (acetonitrile with 2% water and 0.1% formic acidsuggested).• nanoLC priming tool (p/n 801-00003)• 15 mL centrifuge tubestep 1step 2Clean all 50 mL mobile phase reservoirs with appropriate solvents.Close the orange valve on the reservoir by turning it to the perpendicular position.step 3 Fill reservoir A with mobile phase A and reservoir B with mobile phase B.step 4Install both reservoirs and open each valve by rotating to a vertical position.Purging and flushing the pumps are critical operations to get maximum performance from a newinstrument. Purging rapidly replaces the solvent in the pumps while flushing replaces the solvent inthe capillaries connecting the pumps to the sample injector.Mobile PhaseReservoir 2AMobile PhaseReservoir 1BFigure 3-1. Top Panel — Reservoirs for the NanoLC-2D Systemstep 5Select System > Mobile Phases from the main window of the control software to present theMobile Phases dialog box (Figure 3-2).nanoLC Operator’s Manual 611-00012 Revision A1
System Initialization 17step 6 Set the composition for mobile phase A: Enter the correct solvent composition for Mobile Phase A.the A mobile phase will generally be mostly water.step 7 Set the composition for mobile phase B: Enter the correct solvent composition for Mobile Phase B.the B mobile phase will generally be mostly acetonitrile.Figure 3-2. Mobile Phases Windowstep 8 Click the More… button to present the purge and flush settings (Figure 3-3).Figure 3-3. Mobile Phases Window — Advancedstep 9Purge the bubbles from each pump using the priming tool using the following steps:a. Under Purge Settings, select only one pump to purge, i.e. channel 1 A. Set the number ofpurge cycles to 20.b. Click Purge Now. The pump will begin to execute purge cycles. While the pump is purging,insert the priming tool all the way into the pump and depress the check valve.nanoLC Operator’s Manual 611-00012 Revision A1
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Page 3 and 4: iiiTable of ContentsChapter 1. Safe
Page 5 and 6: Chapter 1.Safety and Site Requireme
Page 7 and 8: Safety and Site Requirements 3Do no
Page 9: 1.4.4 Bench Space RequirementSafety
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Page 25 and 26: System Initialization 21step 5step
Page 28 and 29: 24 Operator’s Manual4.2 Disposing
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Page 37 and 38: Chapter 5.Quick-start GuideChapter
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Page 41 and 42: 5.4 Creating an Autosampler MethodQ
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Page 60 and 61: 56 Operator’s ManualAutosampler
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Page 67 and 68: Appendix BExternal InterfaceB.1 Int
Page 69 and 70: External Interface 65B.2 Valve Conn
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External Interface 67B.3.3 Triggeri
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70 Operator’s ManualFigure C-2 sh
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72 Operator’s ManualFigure C-4 sh
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74 Operator’s ManualSelect: Autos
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76 Operator’s ManualDefault metho
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78 Operator’s ManualRun in Synchr
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IndexFlow Rate Verification, 21AFlo
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