NanoLC 1D Plus and 2D System Operator's Manual - Eksigent

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44 Operator’s ManualFigure 5-13. LC Method Editor — Flow Table Tabstep 9 Select the Flow Profile tab to present the Flow Profile dialog box (Figure 5-8)Figure 5-14. LC Method Editor — Flow Profile TabnanoLC Operator’s Manual 611-00012 Revision A1
Quick-start Guide..45step 10 Use the flow profile tab to survey the gradient you created to make sure it is correct. The mobilephase composition can also be set by clicking and dragging points on the graph or by setting the %of A or B in the Profile Editor region.step 11 Once the method is complete, click Save to save the analysis method. Click OK to close the editor.5.7 Creating the Run TableThe Run Table ties together an autosampler and one or more LC method with a sample vial and trayposition. You can also enter descriptive information related to the sample or analysis. This section willcreate a Run Table to run two samples with a trap and elute method.step 1 If not already in the Run Manager, click the Run Manager button located on the Eksigent ControlSoftware’s Acquisition Window. (Figure 5-1).step 2 Create a new, blank Run Table by selecting Edit > Erase Table (Figure 5-15).Figure 5-15. Creating a New Run Tablestep 3 Select File > Save As… and type trap loading in the File name field.step 4 Click Save.step 5 In the first line in the Run Table double click on the Autosampler Method field and select theautosampler method micropickup_1uL_trap loading from the drop down menu.step 6 Next, enter 1 for the tray and A1 for the sample Vial location. Alternatively, vial location can beentered by clicking at the vial on the picture of the vial tray in the Run Manager window.step 7 Double click on the LC Method field and select CH1 Trap Loading. Indicate Channel 1 under theChannel column.step 8 In line 2, leave the autosampler method, tray and vial location blank. Double click on the LC Methodfield and choose CH2 gradient. Indicate Channel 2 under the channel column.step 9 For each sample you wish to run, duplicate these two lines in succession. In Figure 5-16, twosample injections are indicated.nanoLC Operator’s Manual 611-00012 Revision A1
Page 1 and 2: operator’s manualplus systemsyste
Page 3 and 4: iiiTable of ContentsChapter 1. Safe
Page 5 and 6: Chapter 1.Safety and Site Requireme
Page 7 and 8: Safety and Site Requirements 3Do no
Page 9: 1.4.4 Bench Space RequirementSafety
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Page 14 and 15: 10 Operator’s Manual2.7 Installin
Page 16 and 17: 12 Operator’s ManualFigure 2-8. I
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Page 20 and 21: 16 Operator’s ManualRequired tool
Page 22 and 23: 18 Operator’s ManualHold down the
Page 25 and 26: System Initialization 21step 5step
Page 28 and 29: 24 Operator’s Manual4.2 Disposing
Page 30 and 31: 26 Operator’s ManualFigure 4-1. D
Page 32 and 33: 28 Operator’s ManualFigure 4-4. A
Page 34 and 35: 30 Operator’s ManualFigure 4-8. F
Page 37 and 38: Chapter 5.Quick-start GuideChapter
Page 39 and 40: Quick-start Guide..35CloseLoosenGri
Page 41 and 42: 5.4 Creating an Autosampler MethodQ
Page 43 and 44: Quick-start Guide..39Table 5.1. Tem
Page 45 and 46: step 2 To create a new method, type
Page 47: Quick-start Guide..43step 11 The mo
Page 51: Quick-start Guide..47Figure 5-17. A
Page 54 and 55: 50 Operator’s Manual6.2 Calibrati
Page 56 and 57: 52 Operator’s Manual6.4 Troublesh
Page 58 and 59: 54 Operator’s ManualTable 6-1. (c
Page 60 and 61: 56 Operator’s ManualAutosampler
Page 62 and 63: 58 Operator’s Manual6.5 Error Mes
Page 64 and 65: 60 Operator’s ManualA.2 Replaceme
Page 67 and 68: Appendix BExternal InterfaceB.1 Int
Page 69 and 70: External Interface 65B.2 Valve Conn
Page 71: External Interface 67B.3.3 Triggeri
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Page 78 and 79: 74 Operator’s ManualSelect: Autos
Page 80 and 81: 76 Operator’s ManualDefault metho
Page 82 and 83: 78 Operator’s ManualRun in Synchr
Page 89 and 90: IndexFlow Rate Verification, 21AFlo

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