NanoLC 1D Plus and 2D System Operator's Manual - Eksigent

Quick-start Guide..39Table 5.1. Template for Micropickup Injection, Trap Loading Autosampler MethodStep # Operation Volume Parameter Speed Height Description1 Output 1- Off Ensure output 1 relay is in off state2 Output 2- Off Ensure output 2 relay is in off state3 Valve Injector Load Place autosampler valve in loadposition4 Aspirate 19uL Reagent -1 1 0 Aspirate from Reagent 1 vialposition5 Aspirate 1uL Sample 1 (1-5) Aspirate from Sample vial- useappropriate height for sample vial.1= closest to bottom, 5= 5 mm frombottom of vial.6 Aspirate 5uL Reagent -1 1 0 Aspirate from Reagent 1 vialposition7 Output 1-On Send Output 1 relay signal to LCChannel 1, signaling LC Channel 1to start the method8 Wait for input 1 - Low Wait for an input signal (injectionsignal) from Channel 1 beforecontinuing autosampler method.9 Valve Injector Inject Place autosampler valve in the injectposition10 Wait for input 1- High Wait for injection signal from LCChannel 1 to turn off, indicating theloop injection is complete.11 Valve Injector Load Place autosampler valve in the loadposition12 Output 2- On Send Output 2 relay signal to LCChannel 2, signaling LC Channel 2to start the gradient method.13 Dispense 25uL Waste 5 0 Dispense aspirated volume towaste. Total aspirate and dispensevolumes must be equal.14 Needle wash 50uL Perform 50uL needle wash15 End Method endstep 8 To modify method steps, click on the step and change the volume by typing over. Modify theoperation or parameter by clicking on the step and choosing a new value in the drop-down menu.step 9 Add new steps by clicking on the >> arrows to the left of the line. Delete a step by clicking on theX.Note: for an autosampler configured with the standard 25uL syringe,nanoLC Operator’s Manual 611-00012 Revision A1