NanoLC 1D Plus and 2D System Operator's Manual - Eksigent

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12 Operator’s ManualFigure 2-8. Instrument Configuration Windowstep 3 Select the appropriate COM port for the nanoLC from the dropdown list.step 4 Exit from the Control software.step 5 Launch the control software.The Instrument Configuration Window (Figure 2-8) is used to indicate which components areinstalled. It also sets the communications protocol and configures the system to work with otherconnected devices. Several instrument performance parameters are also set in this window.Select System > Instrument Config… from the Control Software’s Acquisition Window to access theInstrument Configuration Window.Make sure that one of the following systems is selected in the Device field.• nanoLC-1D plus• nanoLC-2DThe computer is configured at the factory to use COM1 to communicate with the nano LC system. If theserial cable is connected to a different serial port, change the setting in the on this page to indicate thecorrect COM port.In the Injection Valve field, select Eksigent Internal.If an auxiliary A/D input (such as a UV detector) is connected to the system, specify the voltage rangethe external device will provide under Aux A/D.In the Advanced Options box, type an acceptable idle time after which the system will be shut-downand then check the box.Select the appropriate signal input and output settings based on the requirements of the otherinstruments connected to the Eksigent nano LC system.nanoLC Operator’s Manual 611-00012 Revision A1
System Installation 13Flow Stabilization Limits specifies the degree of flow rate stability required before a gradient willbegin. For most applications, a setting of 100 nL/min should be sufficient. However for high sensitivityapplications, this parameter should be set closer to 20 nL/min.The Pressure Limits box allows for the specification of a maximum system pressure. If the columnpressure exceeds this limit, the run will be automatically stopped and no further injections will takeplace. There is no loss of sample if the system shuts down. For most applications a value of 3500 psi isrecommended.Click OK to go back to the Acquisition Window.2.9 Configuring the nanoLC AutosamplerClick on the Run Manager button to bring up the Run Manager Window for configuring theautosampler (Figure 2-9).Figure 2-9. Run Manager WindowSelect Devices > Autosampler Type > NanoLC-AS1 and the following message will appear:Figure 2-10. Run Manager shutdown promptClick Yes to close the program.Click on Run Manager again, and Select Devices > Autosampler Device Settings. Set the COM portfor the AutosamplernanoLC Operator’s Manual 611-00012 Revision A1
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Page 3 and 4: iiiTable of ContentsChapter 1. Safe
Page 5 and 6: Chapter 1.Safety and Site Requireme
Page 7 and 8: Safety and Site Requirements 3Do no
Page 9: 1.4.4 Bench Space RequirementSafety
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Page 14 and 15: 10 Operator’s Manual2.7 Installin
Page 18 and 19: 14 Operator’s ManualFigure 2-11.
Page 20 and 21: 16 Operator’s ManualRequired tool
Page 22 and 23: 18 Operator’s ManualHold down the
Page 25 and 26: System Initialization 21step 5step
Page 28 and 29: 24 Operator’s Manual4.2 Disposing
Page 30 and 31: 26 Operator’s ManualFigure 4-1. D
Page 32 and 33: 28 Operator’s ManualFigure 4-4. A
Page 34 and 35: 30 Operator’s ManualFigure 4-8. F
Page 37 and 38: Chapter 5.Quick-start GuideChapter
Page 39 and 40: Quick-start Guide..35CloseLoosenGri
Page 41 and 42: 5.4 Creating an Autosampler MethodQ
Page 43 and 44: Quick-start Guide..39Table 5.1. Tem
Page 45 and 46: step 2 To create a new method, type
Page 47 and 48: Quick-start Guide..43step 11 The mo
Page 49 and 50: Quick-start Guide..45step 10 Use th
Page 51: Quick-start Guide..47Figure 5-17. A
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Page 56 and 57: 52 Operator’s Manual6.4 Troublesh
Page 58 and 59: 54 Operator’s ManualTable 6-1. (c
Page 60 and 61: 56 Operator’s ManualAutosampler
Page 62 and 63: 58 Operator’s Manual6.5 Error Mes
Page 64 and 65: 60 Operator’s ManualA.2 Replaceme
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Appendix BExternal InterfaceB.1 Int
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External Interface 65B.2 Valve Conn
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External Interface 67B.3.3 Triggeri
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70 Operator’s ManualFigure C-2 sh
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72 Operator’s ManualFigure C-4 sh
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74 Operator’s ManualSelect: Autos
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76 Operator’s ManualDefault metho
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78 Operator’s ManualRun in Synchr
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IndexFlow Rate Verification, 21AFlo
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NanoLC 1D Plus and 2D System Operator's Manual - Eksigent

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