NanoLC 1D Plus and 2D System Operator's Manual - Eksigent

Quick-start Guide..43step 11 The mobile phase composition can also be set by clicking and dragging points on the graph or bysetting the % of A or B in the Profile Editor region.step 12 Once the method is complete, click Save to save the analysis method.5.6 Creating an LC Method- Channel 2step 1 Open the LC Method editor and type over the name of the method to create a new method. ClickSave.step 2 If you wish, enter any column information appropriate for your experiment. This information isinformation-al and stored with the LC method file.step 3 Click on the Run Conditions tab of the LC Method Editor window to obtain the Run Conditions tab(Figure 5-12).Figure 5-12. LC Method Editor — Run Conditions Tabstep 4 Put a check mark in the Pre-Run Flush column check box and specify a time of 0 minutes to flushthe column using 100% of the initial flow rate conditions.step 5 Select Standard: in the Sample Injection region. This will cause the 10-port valve to be placed inthe inject position for the duration of the Channel 2 run.step 6 Leave the check mark box for Post-Run Flush column empty.step 7 Click on the Flow Table tab to set the gradient parameters.step 8 Enter the gradient parameters you wish to run. Add new steps by clicking on the >> to the left of thetable. Delete steps by clicking on the X. Set the overall flow rate on the right side.nanoLC Operator’s Manual 611-00012 Revision A1