NanoLC 1D Plus and 2D System Operator's Manual - Eksigent

16 Operator’s ManualRequired tools and materials:• Mobile phase reservoirs (p/n 800-00105)• Clean, degassed HPLC-grade mobile phase A (water with 2% acetonitrile and 0.1% formic acidsuggested).• Clean, degassed HPLC-grade mobile phase B (acetonitrile with 2% water and 0.1% formic acidsuggested).• nanoLC priming tool (p/n 801-00003)• 15 mL centrifuge tubestep 1step 2Clean all 50 mL mobile phase reservoirs with appropriate solvents.Close the orange valve on the reservoir by turning it to the perpendicular position.step 3 Fill reservoir A with mobile phase A and reservoir B with mobile phase B.step 4Install both reservoirs and open each valve by rotating to a vertical position.Purging and flushing the pumps are critical operations to get maximum performance from a newinstrument. Purging rapidly replaces the solvent in the pumps while flushing replaces the solvent inthe capillaries connecting the pumps to the sample injector.Mobile PhaseReservoir 2AMobile PhaseReservoir 1BFigure 3-1. Top Panel — Reservoirs for the NanoLC-2D Systemstep 5Select System > Mobile Phases from the main window of the control software to present theMobile Phases dialog box (Figure 3-2).nanoLC Operator’s Manual 611-00012 Revision A1