NanoLC 1D Plus and 2D System Operator's Manual - Eksigent

More documents

Recommendations

Info

34 Operator’s ManualFigure 5-1. Acquisition Window5.2 Purging and Flushing with New SolventsIf solvent has been sitting for more than 2 weeks, the solvent should be replaced with fresh solvent,then purged and flushed. If the solvent is less than two weeks old, proceed to step 5.3.step 1 To discard old solvent in the reservoir bottles, one of two methods can be used: remove the bottleand pour out the old solvent, or purge the old solvent through to waste (Figure 5-2).nanoLC Operator’s Manual 611-00012 Revision A1
Quick-start Guide..35CloseLoosenGripstopcockpullupEmptyReplaceFigure 5.2- Emptying Solvent Reservoirsstep 2 Pour new mobile phase into the bottle. Purge the HPLC at least 10 times. Depress the check valvewhile purging to dislodge bubbles in the pump head, as shown in figure 3.3.1.step 3 After the pump is thoroughly purged, flush. Select System > Mobile Phases from the main controlwindow. Click More and flush the system 100uL on Channel 1 and Channel 2. See section 3.2 formore information on flushing.5.3 Equilibrating the SystemThe Control software’s Direct Control window (Figure 5-3) can be used to equilibrate the systemfollowing system power-up, a change of solvent or change of column. The injection valve can also betoggled between load and inject positions to flush the injection valve loop and interconnecting ports.Select System > Direct Control from the main Control software Acquisition Window.nanoLC Operator’s Manual 611-00012 Revision A1
Page 1 and 2: operator’s manualplus systemsyste
Page 3 and 4: iiiTable of ContentsChapter 1. Safe
Page 5 and 6: Chapter 1.Safety and Site Requireme
Page 7 and 8: Safety and Site Requirements 3Do no
Page 9: 1.4.4 Bench Space RequirementSafety
Page 12 and 13: 8 Operator’s Manualstep 5step 6st
Page 14 and 15: 10 Operator’s Manual2.7 Installin
Page 16 and 17: 12 Operator’s ManualFigure 2-8. I
Page 18 and 19: 14 Operator’s ManualFigure 2-11.
Page 20 and 21: 16 Operator’s ManualRequired tool
Page 22 and 23: 18 Operator’s ManualHold down the
Page 25 and 26: System Initialization 21step 5step
Page 28 and 29: 24 Operator’s Manual4.2 Disposing
Page 30 and 31: 26 Operator’s ManualFigure 4-1. D
Page 32 and 33: 28 Operator’s ManualFigure 4-4. A
Page 34 and 35: 30 Operator’s ManualFigure 4-8. F
Page 37: Chapter 5.Quick-start GuideChapter
Page 41 and 42: 5.4 Creating an Autosampler MethodQ
Page 43 and 44: Quick-start Guide..39Table 5.1. Tem
Page 45 and 46: step 2 To create a new method, type
Page 47 and 48: Quick-start Guide..43step 11 The mo
Page 49 and 50: Quick-start Guide..45step 10 Use th
Page 51: Quick-start Guide..47Figure 5-17. A
Page 54 and 55: 50 Operator’s Manual6.2 Calibrati
Page 56 and 57: 52 Operator’s Manual6.4 Troublesh
Page 58 and 59: 54 Operator’s ManualTable 6-1. (c
Page 60 and 61: 56 Operator’s ManualAutosampler
Page 62 and 63: 58 Operator’s Manual6.5 Error Mes
Page 64 and 65: 60 Operator’s ManualA.2 Replaceme
Page 67 and 68: Appendix BExternal InterfaceB.1 Int
Page 69 and 70: External Interface 65B.2 Valve Conn
Page 71: External Interface 67B.3.3 Triggeri
Page 74 and 75: 70 Operator’s ManualFigure C-2 sh
Page 76 and 77: 72 Operator’s ManualFigure C-4 sh
Page 78 and 79: 74 Operator’s ManualSelect: Autos
Page 80 and 81: 76 Operator’s ManualDefault metho
Page 82 and 83: 78 Operator’s ManualRun in Synchr
Page 89 and 90:
IndexFlow Rate Verification, 21AFlo
show all

NanoLC 1D Plus and 2D System Operator's Manual - Eksigent

Create successful ePaper yourself

Delete template?

Save as template?