NanoLC 1D Plus and 2D System Operator's Manual - Eksigent

System Initialization 21step 5step 6step 7Open the Direct Control Window (Fig. 3-5) by clicking System > Direct Control from the Controlsoftware’s Acquisition Window.Set solvent A and B to 50/50 and Total flow rate to 20 µL/min. (for NanoLC-1D+, use 100%A).Click Start to flush the valve ports and capillary. Flush for 10 minutes.Figure 3-5. Direct Control Window — Flushing CH1 Autosampler Connectionstep 8Stop the flow by clicking Stop.3.6 Verifying the Flow RateBefore operating the system it is suggested that you verify that the flow rate is properly calibrated. Thisis done by measuring the time it takes to move a liquid front through a graduated capillary of knownvolume.Required tools:• Flow calibration assembly (p/n 801-00002) for high flow rate channel (includes 20 µL pipettes)• Flow calibration assembly (p/n 910-00006) for low flow rate channel (includes 5 µL graduatedpipettes)step 1 Re-initialize the pressure transducers (see section 4.5).step 2 Attach the appropriate flow calibration assembly to the outlet of channel 1 or 2.step 3Select System > Direct Control from the Eksigent Control Software Acquisition Window.step 4 Set channel A to 100% and an appropriate flow rate for that channel (Figure 3-6).• 5 µL/min for high flow channel• 500 nL/min for low flow channelnanoLC Operator’s Manual 611-00012 Revision A1