characteristics under study have shown a high level (V > 30 %) of variability indicating the highestdegree of varietal distinctions.Table 1. Averaged quantity indicators of the biochemical structure of fruits (dry weight) andvariation coefficients in a long-term cycle of supervision for a varietal row of Vacciniumcorymbosum L.2006 2007 2008PeriodIndex mean V, % mean V, % mean V, %averageV, %Soluble solids, % of freshfruit13.9 12.8 13.9 10.1 14.1 12.1 11.7Free organic acids, % 5.7 36.4 3.8 45.7 6.7 39.1 40.4Ascorbic acid, mg % 601.2 20.3 426.6 26.3 604.8 30.3 25.6Glucose, % 5.34 16.4 4.49 15.8 4.96 27.5 19.9Fructose, % 18.74 2.9 14.22 3.9 7.26 22.3 9.7Sucrose, % 3.19 19.8 2.08 20.2 0.56 22.1 20.7Sum of soluble sugars, % 27.25 4.1 20.79 5.1 12.79 6.8 5.3Fructose/ Glucose 3.6 19.2 3.2 16.5 1.7 46.0 27.2Monose/Disaccharide 7.9 19.4 9.4 19.8 22.7 21.5 20.2Sugar-acid index 5.4 31.2 6.5 39.5 2.5 75.9 48.9Hydropectin, % 1.98 24.3 2.18 23.8 2.37 18.0 22.0Protopectin, % 3.45 22.4 2.60 19.8 3.35 21.0 21.1Sum of pectins, % 5.43 16.5 4.77 19.2 5.71 17.8 17.8Protopectin/Hydropectin 1.8 33.8 1.2 22.9 1.4 17.1 24.6Anthocyanins, mg % * 7.6 45.1 2.0 91.5 17.1 35.5 57.4Leucoanthocyanins, mg% 17.4 23.2 12.1 20.6 24.1 29.1 24.3Sum of anthocyanicpigments, mg %25.0 19.7 14.1 21.5 41.2 27.1 22.8Catechines, mg % 984.3 20.2 923.4 27.6 570.1 15.4 21.1Flavonols, mg % 1766.6 5.5 1626.0 11.0 1890.6 12.2 9.6Flavonols/Catechines 1.9 27.1 1.9 36.3 3.4 20.9 28.1Sum of bioflavonols, mg % 2776.0 6.9 2563.5 11.7 2501.8 9.2 9.3Phenol-carboxylic acids,mg %781.4 18.0 800.3 24.4 787.3 24.3 22.2Benzoic acid, % 1.15 6.1 1.11 18.2 1.18 14.3 12.9Tannins, % 1.21 19.5 1.72 23.2 1.83 16.3 19.7Fat oils, % 3.61 29.9 3.17 13.0 3.25 21.9 21.6Nitrogen, % 0.76 15.1 0.91 16.2 1.10 10.3 13.9Phosphorus, % 0.17 24.0 0.14 11.5 0.14 13.0 16.2Potassium, % 0.53 10.0 0.57 8.7 0.76 11.3 10.0Calcium, % 0.31 10.0 0.42 4.9 0.40 4.9 6.6Magnesium, % 0.09 11.9 0.08 8.8 0.11 6.8 9.2* mg% - mg of substance in 100 g of dry weight of sample.The variability level of a particular characteristic only in some cases remained stable throughoutthe whole 3-year period of observation. For example, such stability of a low variability level wastypical for the parameters of the accumulation of solid, flavonols, potassium, calcium, magnesium,the total amount of sugars and the sum of bioflavonols in fruits whereas the parameters of theaccumulation of fructose, benzoic acid, fat oils, nitrogen and phosphorus in fruits were notable forlow variability only during one or two seasons. Similarly, the high level of genotypic variabilityduring the whole period of observation was typical for the parameters of the accumulation of freeorganic acids, anthocyanins and the values of a sugar-acid index whereas for the ascorbic acidcontent it has been notable only in 2008. Moreover, even within a particular area of variability ofthe characteristics analyzed there have been identified some essential interseasonal distinctions.This fact unequivocally indicates a dependence of the genotypic variability of quantity indicators ofbiochemical composition of Vaccinium corymbosum L. fruits on the hydrothermal mode of itsformation period.106
In our opinion, the best estimate about the degree of variability of the indicators in the varietal rowcan be given by the averaged values of variation coefficients calculated within a 3-year period ofobservation. In this case the characteristics under study can be provisionally divided into 3 groupsin an increasing order of genotypic variability, and, hence, intensification of varietal distinctions:1 – with small variability (V = 5.3 – 13.9 %) – the content of solid, fructose, total of solublesugars, flavonols, total bioflavonols, benzoic acid, nitrogen, potassium, calcium and magnesium infruits;2 – with average variability (V = 16.2 – 25.6 %) – the content of phosphorus, ascorbic acid,glucose, sucrose, hydropectin, protopectin, leukoanthocyanins, catechines, phenol-carboxylic acids,tannins and fat oils in fruits;3 – with high variability (V = 40.4 – 57.4 %) – the content of free organic acids,anthocyanins proper and values of a sugar-acid index in fruits.ConclusionThe biochemical screening of the fruits of 16 representatives within three groups of introducedblueberry cultivars with different terms of ripening (early-ripening, mid-ripening and late-ripening)according to 30 indicators specifying the content of some organic acids, carbohydrates,bioflavonols, terpenoids and chemical elements under vegetative seasons of 2006 – 2008 made itpossible to reveal the essential intervarietal distinctions in the accumulation of certain compoundsin the dry weight of fruits, confirmed by a wide range of changes of their quantitativecharacteristics. On the basis of the comparative analysis of the averaged variation coefficientscalculated for a varietal row of Vaccinium corymbosum L. during a long-term cycle of observationit has been established that the parameters of accumulation of solids, fructose, total soluble sugars,flavonols, total bioflavonols, benzoic acid, nitrogen, potassium, calcium and magnesium in fruitscan be characterized by the smallest degree of genotypic variability and, consequently, ofintervarietal distinctions, whereas the content of free organic acids, anthocyanins proper and thevalues of a sugar-acid index in fruits are characterized by the highest degree of genotypicvariability.References1. GOST 8756.2-82, (1982) Methods of definition of solids. М: Publishing house of standards, 5 p. (InRussian).2. The State pharmacopoeia of the USSR (1987) Vol.1. The general methods of the analysis, М: Medicine,pp.286-287. (In Russian).3. Ermakov A.I., et al. (1987) Methods of biochemical research of plants. М, VO Agropromizdat,. 430 p.(In Russian).4. Zavadskaja I.G., Gorbacheva G. I and Mamushina N.S. (1962) Quantitative definition of carbohydratesby resorcinol and aniline-phthalate methods by means of a paper chromatography. In: Kretovich V.L.(eds) The Technique of a quantitative paper chromatography of sugars, organic acids and amino acidsat plants, Moscow-Leningrad, Publishing house of AS of the USSR,. pp. 17-26. (In Russian).5. Zaprometov M. N. (1964) Biochemistry of catechines, Moscow, Science, 325 p. (In Russian).6. Kalebin M. I, and Kolesnik A.A. (1949) Research of fresh fruits, vegetables and products of theirprocessing. In: Tserevitinov F.V. (etc) Research of foodstuff, Мoscow, Gostorgizdat, pp. 218-245. (InRussian).7. Mzhavanadze V.V., Targamadze I.L., and Dranik L.I. (1971) Quantitative definition of chlorogenic acidin leaves of a bilberry Caucasian V. arctostaphylos L.) Proc. of АS of GSSR, Vol. 63, N 1, pp. 205-210.(In Russian).8. Sapunov V.А., and Fedunjak I.I. (1958) Methods of an estimation of forages and the zootechnicalanalysis. Minsk, 190 p. (In Russian).9. Sarapuu L., and Mijdla H. (1971) The phenolic constituents of an apple-tree. Uch. West Tart. SU, N 256,pp. 111-113.10. Sennov S.N., and Kovjazin V.F. (1990) Forestry: the Manual, Leningrad, LTA, 91 p. (In Russian).11. Simonjan A.V., Shinkarenko A.L., and Oganesyan E.T. (1972) Quantitative definition of triterpenes inplants of Thymus sort. Chemistry of natural compounds, N 3, pp.293-295. (In Russian).12. Skorikova J. G, and Shaftan E.A. (1968) Technique of anthocyanins definition in fruits and berries. In:Kefeli V.I. (etc) Proc. 3 Un. Seminar on biologically active (medical) substances of fruits and berries.Sverdlovsk, pp. 451-461. (In Russian).107
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Conference Organizing CommitteeChai
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15 Pormale J., Osvalde A. and Nolle
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were established in 1985. Nowadays,
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10,1-15 ha7%15,1-20 ha7%< 20,1 ha0%
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In less than half the surveyed farm
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economical and biochemical characte
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investigated European cranberry acc
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fruit of V. opulus has different am
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As several authors have stated (Koz
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KopsavilkumsVaccinium ăints kultū
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maintained in a mist chamber with v
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period and produce vigorous vegetat
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38. Marcotrigiano M. and McGlew S.P
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of changes in the typological struc
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fall from 2 to 3 and that for heath
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HIGHBUSH BLUEBERRY BREEDINGAUGSTKR
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Southern and Intermediate highbush
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and anatomically they belong to fal
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The levels of flavonols are more co
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21. Polashock J.J., Griesbach R.J.,
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Figure 1. A general scheme of the N
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5. Åkerström A., Forsum Å., Rump
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species and studying the efficiency
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Thus, it has been determined that t
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CHEMICAL COMPOSITION OF HIGHBUSH BL
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