Agronomijas v stis - Latvijas Lauksaimniecības universitāte

IN VITRO PROPAGATION OF SEVERAL VACCINIUM CORYMBOSUM L. ANDVACCINIUM VITIS-IDAEA L. CULTIVARSVAIRĀKU VACCINIUM CORYMBOSUM L. UN VACCINIUM VITIS-IDAEA L. ŠĖIRĥUIN VITRO PAVAIROŠANAMária Gabriela Ostrolucká 1 , Alena Gajdošová 1 , Emilia Ondrušková 2 and GabrielaLibiaková 11Institute of Plant Genetics and Biotechnology SAS, Akademická 2, P.O.Box 39A, 950 07 Nitra, SlovakRepublic; e-mail: gabriela.ostrolucka@savba.sk2Institute of Forest Ecology, Branch for Wood Plants Biology Nitra, Akademická 2, P.O.Box 39A, 950 07Nitra, Slovak Republic; e-mail: ondruskova@savzv.skAbstractIn the paper the efficient in vitro regeneration and propagation systems in several Vacciniumcorymbosum L. and Vaccinium vitis-idaea L. cultivars are described. It was found that shootregeneration ability is highly dependent on the cultivar and cytokinin applied. Zeatin showed itselfto be efficient for axillary and adventitious shoot induction in both species. A zeatin concentrationof 2 mg l -1 was the best for axillary shoot regeneration in V. corymbosum, while zeatin in aconcentration of 0.75 - 1.5 mg l -1 was suitable for V. vitis-idaea cultivars. Multiple adventitiousshoot formation on leaf explants of V. corymbosum occurred on the medium with 0.5 mg l -1 zeatin.In V. vitis-idaea indirect shoot regeneration was observed in cv. ´Red Pearl´ after transfer of thecalli on the medium with 0.5 mg l -1 zeatin. For long-term proliferation of in vitro regeneratedaxillary or adventitious shoots an AN medium with 0.5 mg l -1 zeatin was successfully used.Satisfactory rooting of isolated microshoots was achieved in vitro on an AN medium supplementedwith 0.8 mg l -1 IBA and 0.8 g l -1 charcoal.KopsavilkumsŠajā rakstā ir apskatīta efektīva in vitro reăenerācijas un pavairošanas sistēma vairākām Vacciniumcorymbosum L. un Vaccinium vitis-idaea L. šėirnēm. Dzinumu reăenerācijas spēja ir atkarīga nošėirnes un apstrādes ar citokinīniem. Zeatīns bija efektīvs, lai veicinātu sānu un adventīvo dzinumuaugšanas veidošanu. V. corymbosum sāndzinumu veidošanai piemērotākā zeatīna koncentrācija bija2 mg l -1 , bet V. vitis-idaea šėirnēm piemērotāka bija 0.75 - 1.5 mg l -1 zeatīna koncentrācija. Barotnēar 0.5 mg l -1 zeatīnu novērota masveidīga adventīvo dzinumu veidošanās V. corymbosum lapueksplantiem. Netieša dzinumu reăenerācija tika novērota V. vitis-idaea šėirnei ‘Red Pearl’ pēckallusa pārnešanas uz barotni ar 0.5 mg l -1 zeatīnu. Zeatīns 0.5 mg l -1 koncentrācijā tika sekmīgipielietots AN vidē, lai nodrošinātu ilgstošu in vitro reăenerēto sānu un adventīvo dzinumuproliferāciju. Apmierinoša izolēto mikrodzinumu apsakĦošanās tika sasniegta AN vidē, kaspapildināta ar 0.8 mg l -1 indolilsviestskābes un 0.8 g l -1 kokogĜu.Key words: Vaccinium corymbosum L., Vaccinium vitis-idaea L., axillary shoot and adventitiousshoot regenerationAbbreviations: AN – Anderson medium; IBA – indole-3-butyric acid; TDZ - thidiazuron (Nphenyl-N1-1,2,3,-thiadiazol-5-ylurea);2-iP (N6-[2-Isopentenyl]adenine)IntroductionThe Vaccinium corymbosum L. and Vacinium vitis-idaea L. represent commercially important andbiologically valuable small fruits (Song and Sing, 2004) desirable for cultivation in various parts ofthe world. Production of high quality plants in large amounts is needed for commercial plantationestablishment. In this respect, in vitro plant regeneration from dormant buds and throughadventitious organogenesis enables effective mass production of plant material and to the createnecessary conditions for the application of genetic engineering. Several papers have describedmicropropagation methods for these economically important species (Shibli and Smith, 1996;Jaakola et al., 2001; Debnath and McRae, 2002; Ostrolucká et al., 2002; Cao et al., 2003;75