Agronomijas v stis - Latvijas Lauksaimniecības universitāte

Figure 2. Axillary shoot regeneration in V. vitis-idaea cv.’Koralle’ on AN medium with 0.75 mg l -1zeatin (A) and cv.’Red Pearl’ on AN medium with 15 mg l -1 2-iP (B), control (C).Satisfactory rooting of isolated microshoots in V. corymbosum L. and V. vitis-idaea L. wasachieved on an AN medium supplemented with 0.8 mg l -1 IBA and 0.8 g l -1 charcoal. In V.corymbosum the percentage of rooting was 95 %, 90 % and 80 % in cv. ‘Berkeley’, ‘Bluecrop’ and‘Brigitta’, respectively. In V. vitis-idaea the rooting percentage reached 60 % and 40 % in ‘ReadPearl’ and ‘Koralle’, respectively. Rooted plantlets transplanted into peat substrate weresubsequently acclimatized under greenhouse conditions without any special light treatment, withregular irrigation, in containers at the beginning (2 weeks) covered by lid, later opened. Plantlets inlength 80 – 100 mm (after 2 months) were replanted into bigger containers (120 mm in diameter)and placed under open-air conditions. Transfer of regenerants from in vitro to ex vitro conditionsand their acclimatization was successful, as almost 80-90 % of transferred plants survived.Figure 3. Adventitious shoot proliferation in V. vitis-idaea cv.’Red Pearl’derived from leaf tissuecultivated on AN medium with 0.5 mg l -1 zeatin (A, B). High shoot proliferation in V. corymbosumcv. ‘Bluecrop’ on AN medium with 0.5 mg l -1 zeatin (C).In conclusion we can state, that efficient cloning protocols were developed for the several selectedblueberry and lingonberry cultivars, which enable large-scale propagation of high quality, true totype plants for economic and growing needs.AcknowledgementsThe work was supported by Slovak Grant Agency VEGA, project no. 2/0004/08 and MVTS-COST863 Action.References1. Anderson W.C. (1980) Tissue culture propagation of red and black raspberries, Rubus idaeus and R.occidentalus. Acta Hort. (ISHS), 112, pp. 124-132.2. Cao X. and Hammerschlag F.A. (2000) Improved shoot organogenesis from leaf explants of highbushblueberry. Hort. Science, 35, pp. 945-947.3. Cao X., Fordham I., Douglass L. and Hammerschlag F. (2003) Sucrose level influencesmicropropagation and gene delivery into leaves from in vitro propagated highbush blueberry shoots.Plant Cell Tiss. Org. Cult., 75, pp. 255-259.79