4. Kozłowski J, Buchwald W, Mścisz A et al. Uprawa borówki i Ŝurawiny (z elementami ekologii)(Growing of Blueberries and Cranberries (With Ecological Aspects), 2006, pp.229-235. (In Polish).5. Panteev A., Batchilo A., Grakovich Z. (1995) The Breeding of Chaenomeles japonica Lindl (JapaneseQuince), Cerasus tomentosa Wall. (felt cherry), and Viburnum opulus L. (Snowball tree) in the republicBelarus. Acta Horticulturae. 390, pp.133-136.6. Shwartz J., Medrek T. (1968) Antifungal Properties of Cranberry Juice. Applied Microbiology, 16(10),pp.1524-1527.7. Smolarz K. (2003) Uprawa borówki i Ŝurawiny (Growing of Bluebeeries and Cranberries), pp.182-187.(In Polish).8. Velioglu Y.S., Ekici L., Poyrazoglu E.S. (2006) Phenolic composition of European cranberrybush(Viburnum opulus L.) berries and astringency removal of its commercial juice. International Journal ofFood Science and Technology. 41, pp.1011-1015.9. Ермаков А., Арасимович В. (1987) Методы биохимических исследований растений (The Methodsof Plants Biochemical Investigation). 136 p. (In Russian).10. Куденков М., Чурканенко Н.. (1998) Varieties of guelder rose and sea buckthorn released in Russia.Садовотство и виноградовотство (Horticulture and Viticulture). 2, pp.20-21. (In Russian).11. Лобанова А., Сысолятин С., Сакович Г., Зимина И. (1999) Масло плодов Viburnum opulus L. (Oilsof Viburnum opulus L. fruits) Химия растительного сырья (Chemistry of Vegetatyve Raw Matherial).4, pp.101-103. (In Russian).12. Оводова Р., Головченко В., Попов С., Шашков А. (2000) The isolation, preliminary study of structureand physiological activity of water-soluble polysaccharides from squeezed berries of snowball treeviburnum opulus. Биоорганическая химия (Bioorganic Chemistry). 26(1), pp.61-67. (In Russian).13. Рупасова Ж., Василевская Т. (1999) Клюква крупноплодная в Беларуси (Large Fruited Cranberry inBelarus), pp. 6-31. (In Russian).PROPAGATION AND CULTIVATION OF VACCINIUM SPECIES AND LESSKNOWN SMALL FRUITSVACCINIUM ĂINTS SUGU UN MAZĀK ZINĀMO AUGěAUGUPAVAIROŠANA UN AUDZĒŠANASamir C. DebnathAtlantic Cool Climate Crop Research Centre, Agriculture and Agri-Food Canada,P.O. Box 39088, 308 Brookfield Road, St. John’s, NL A1E 5Y7, Canada,e-mail: Samir.Debnath@AGR.GC.CAAbstractThe production of Vaccinium species crops has recently been the subject of much interest globallybecause of an improved understanding of the important role of dietary fruit in maintaining humanhealth. Cloudberry (Rubus chamaemorus L., family Rosaceae), a less known small fruit ofmedicinal importance, and the Vaccinium species are genetically heterozygous and do notreproduce progeny from seed that are similar to the seed parent. Tremendous progress in planttissue culture, resulting in great advances in micropropagation, has occurred in these crops. Ofparticular significance has been the evolution of the technology permitting multiplication of theseplants through bioreactor micropropagation. The in vitro morphogenesis seems to be highlydependent on the plant growth regulators and media used for the culture, which is again genotypespecific. Although the automation of micropropagation in bioreactors has been advanced as apossible way of reducing propagation cost, optimal plant production depends upon a betterunderstanding of the physiological and biochemical responses of plants to the signals of the culturemicroenvironment and an optimization of specific physical and chemical culture conditions tocontrol the morphogenesis of berry plants in liquid culture systems. Clonal fidelity can be a seriousproblem and molecular strategies have been developed to reduce the variation to manageablelevels. The paper focuses on conventional and bioreactor systems used for the in vitro culture of theVaccinium species and cloudberry, cultivation of micropropagules and the employment ofmolecular markers in micropropagated plants for the assessment of genetic fidelity, uniformity,stability and true-to-typeness among donor plants and tissue culture regenerants.22
KopsavilkumsVaccinium ăints kultūraugu audzēšanai pēdējā laikā visā pasaulē pievērsta pastiprināta uzmanība,jo arvien labāk tiek izprasta šo augĜu diētiskā nozīme cilvēka veselības saglabāšanai. Lācenes(Rubus chamaemorus L., Rosaceae dzimta) – mazāk zināmi augĜi ar medicīnisku nozīmi unVaccinium ăints sugas ir ăenētiski heterozigoti un, pavairojot ar sēklām, nedod vecākiem līdzīguspēcnācējus. Milzīgais progress augu šūnu kultūru izpētē ir izraisījis arī lielus uzlabojumus šokultūraugu mikropavairošanā. Īpaši nozīmīga ir bijusi tādas tehnoloăijas attīstība, kas Ĝauj šo augumikropavairošanu veikt bioreaktorā. Morfoăenēze in vitro apstākĜos ir Ĝoti atkarīga no kultivēšanāizmantotajiem augu augšanas regulatoriem un barotnes, kas ir atkarīga no genotipa. Lai ganmikropavairošanas automatizācija bioreaktoros ir attīstīta kā iespējamais pavairošanas izmaksusamazināšanas ceĜš, optimāla stādu ražošana ir atkarīga no labākas izpratnes par augu bioėīmiskoun fizioloăisko reakciju uz mikrovides signāliem un specifisku fizikālu un ėīmisku kultivēšanasapstākĜu optimizācijas, lai kontrolētu ogaugu morfoăenēzi šėidrās kultivēšanas sistēmās. Klonālāmainība var būt nopietna problēma, tāpēc ir izveidotas molekulāras metodes, lai samazinātu šomainību līdz iespējami zemākam līmenim. Šajā rakstā lielākā uzmanība pievērstakonvencionālajām un bioreaktora sistēmām Vaccinium ăints kultūraugu un lāceĦu in vitropavairošanai, mikropavairoto augu kultivēšanai un molekulāro marėieru izmantošanai ăenētiskāsatbilstības, viendabīguma, stabilitātes un autentitātes novērtēšanai starp donora augiem un audukultūrā iegūtajiem stādiem.Key words: cloudberry, propagation, micropropagation, in vitro, genotype.IntroductionThe genus Vaccinium L. (family: Ericaceae) contains about 400 species, and one or more speciesare native to all continents except Antarctica and Australia (Vander Kloet, 1988; Ballington, 2001).It is typically characterized as having fleshy, more-or-less edible fruits with very high levels ofvitamin C, cellulose, pectin and anthocyanins possessing antitumor, antiulcer, antioxidant andantiinflammatory activities (Wang et al., 1999). The proanthocyanidins in cranberries have beenshown to help prevent urinary tract infections through reduced adhesion of uropathogenicEscherichia coli (Howell et al., 2005). Lingonberry fruits and leaves are used to lower cholesterollevels and treat stomach disorders, rheumatic diseases, and bladder and kidney infections (Novelli,2003). Blueberry (Vaccinium spp.), cranberry (V. macrocarpon Ait.), and lingonberry (V. vitisidaeaL.) are three commercially cultivated Vaccinium fruit crops of economic importance.Although the majority of cultivated blueberry hectarage is in the United States and in Canada, theyare also grown commercially in Europe, Asia, Africa, Australia, New Zealand and South America(Lehnert, 2008). While the leading countries in cranberry production are the United States, Canada,Latvia and Poland; its culture has also shown promise in Austria, Germany and Russia(http:////aesop.rutgers.edu/~bluecran/cranberrypage.htm).Commercial lingonberry production primarily involves the harvesting of berries from wildpopulations in northern Europe, Asia and North America, with cultivated production still in itsinfancy compared with cranberries and blueberries (Ballington, 2001). The cloudberry (Rubuschamaemorus L., family Rosaceae), a less known small fruit crop, is a boreal circumpolar,rhizomatous dioecious perennial herb common to bogs. The berries and leaves of cloudberry arerich in vitamin C and tannins, and possess high ellagic acid content (Amakura et al., 2000).Cloudberries are used medicinally to treat scurvey and diarrhea in traditional medicine (Thiem,2003).Although conventional vegetative propagation methods by cuttings or rhizome divisions aresuccessful in these species, the micropropagation of selected germplasm can potentially multiplyplants more rapidly than traditional propagation methods. Various culture conditions, basal mediaand growth regulators have been investigated for the micropropagation of these crops on semisolidgelled media (for review, please see Debnath, 2003a, 2006a, 2007a).However, these techniques are difficult to automate and the production cost is high. Automatedbioreactors for large scale production of micropropagated plants are important for themicropropagation industry. Bioreactors are self-contained, sterile environments which capitalize onliquid nutrient or liquid/air inflow and outflow systems, designed for intensive culture and control23
- Page 3 and 4: Conference Organizing CommitteeChai
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isothermically at 70°C for 5 min,
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IN VITRO PROPAGATION OF SEVERAL VAC
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16BM ean N o. of shoots/explant1412
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Figure 2. Axillary shoot regenerati
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evaluate the blueberries supply wit
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espectively). It should be stressed
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lueberry appear to play a conclusiv
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15. Reimann C., Kollen F., Frengsta
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each type, and for comparison sampl
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the mean. Kisgyır 1 sample has the
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13. Porpáczy A. (1999) A húsos so
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was medium (0.014 - 0.017 g kg -1 s
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‘Salaspils Ražīgā’. Vigorous
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KopsavilkumsEiropas melleĦu (Vacci
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Figure 2. Chemometric PCA of 32 blu
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References1. Baloga D.W., Vorsa N.,
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obtained from fruits of black choke
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In our opinion, the best estimate a
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cuttings also varies markedly with
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shoots shorter than 10 mm were not
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14. Ostrolucka M.G., Gajdosova A, L
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„Metos RG-350” (http://www.meto
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500480Phenols,mg 100g -146044042040
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SHORT INFORMATION ABOUT THE HISTORY
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Evaluation of cultivars. After the
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the number of pistils (female clone
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Table 2. Number of flowers per harv
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ResultsFirst time upright dieback i
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grew rapidly on PDA at 20 - 24 o C.
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Figure 9. Conidia of Physalospora v
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References1. CABI, EPPO, (1997) Dia
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Results und DiscussionBerries were
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In literature Caruso eds. and Гop
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the total area under a cranberry ma
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Skilled works on development of the
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Tika atrastas dažas būtiskas ats
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appears to maintain a quite low lev
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8. Garkava - Gustavson L.,Persson H