(Statistical analysis of agronomic evaluations by using the programmes ANOVA, STAT, SPLIT-PLOTfrom SELEKCIJA and IRRISTAT). Akademija, 56 p. (In Lithuanian).12. Vorsa N., Polashock J., Cunningham D., Roderick R. and Howell A. (2002). Evaluation of fruitchemistry in cranberry germplasm: potential for breeding varieties with enhanced health constituents.Acta Horticulturae, 574, pp.215-219.13. Zeldin E.L., McCown B.H., Krueger C.G. and Reed J.D. (2007). Biochemical characterization andbreeding of American cranberry for increased health benefits. Acta Horticulturae, 744, pp.253-258.THE EVALUATION OF EUROPEAN CRANBERRYBUSH (VIBURNUM OPULUS) FORBREEDING IN LITHUANIAIRBENES (VIBURNUM OPULUS) PIEMĒROTĪBA AUDZĒŠANAI LIETUVĀRemigijus Daubaras, Laima Česonien÷Kaunas Botanical Garden of Vytautas Magnus University,Z.E.Zilibero 6, LT-46324, Kaunas, Lithuania, e-mail: l.cesoniene@bs.vdu.lt,AbstractThe investigations were carried out at the European cranberrybush (Viburnum opulus L.) collectionat the Kaunas Botanical Garden of Vytautas Magnus University in 1999 – 2005. The biologicalpeculiarities of cranberrybush cultivars and clones were estimated according to the usual methodsof the evaluation of horticulture plants. Reliable differences were determined between accessionswith respect to productivity, the number of fruit per cluster and the mean weight of the fruit. Theinvestigations of the biochemical composition of the fruit of different cultivars and clones revealedsignificant differences in the amounts of phenolic compounds (anthocyanins and flavonols),benzoic, and ascorbic acids. The accession of Lithuanian origin P1 was typical of the largestamount of ascorbic acid (46.1 mg 100 g -1 ). The fruit of the cultivar ‘Leningradskaja Otbornaja’accumulated the largest amounts of anthocyanins. The cultivars ‘Krasnaja Grozd’ as well as theclone P2 had the largest benzoic acid amounts. The value of the most productive Europeancranberrybush accessions were determined by the number of fruit in a cluster. The results of thebiochemical investigations corroborated that the selection of European cranberrybush accessions inrespect to benzoic acid, anthocyanins and ascorbic acid amounts could be advisable.KopsavilkumsIzmēăinājumi tika veikti irbeĦu (Viburnum opulus L.) kolekcijas stādījumā Vitauta Magnusuniversitātes KauĦas botāniskajā dārzā no 1999. līdz 2005. gadam. Tika noteiktas dažādu irbeĦušėirĦu un klonu bioloăiskās īpašības. Atrastas būtiskas atšėirības starp dažādu paraugu ražību,augĜu skaitu ėekarā un augĜa vidējo masu. AugĜu bioėīmiskā sastāva pētījumi uzrādīja būtiskasatšėirības starp dažādu šėirĦu un klonu fenolu (antocianīni un flavonoli), benzoskābes unaskorbīnskābes saturu. Lietuvas izcelsmes paraugs P1 saturēja visvairāk askorbīnskābes – 46.1 mg100g -1 . Šėirnes ‘Leningradskaja Otbornaja’ augĜi saturēja visvairāk antocianīnu, bet šėirne‘Krasnaja Grozd’ un klons P2 – visvairāk benzoskābes. Produktīvāko irbenes vērtību noteica augĜuskaits ėekarā. Bioėīmiskā sastāva pētījumu rezultāti rāda, ka būtu ieteicams veikt irbeĦu selekcijuattiecībā uz askorbīnskābes, benzoskābes un antocianīnu saturu.Key words: evaluation, biochemical composition, cultivars, productivity, breeding.IntroductionThe species of genus Viburnum L. such as Viburnum opulus L. (European cranberrybush), V.trilobum Marsh. (American cranberrybush) as well as V. sargentii Koehne are widely used intraditional and folk medicine. The European cranberrybush (Viburnum opulus L.) is a native plantin Lithuania which is widely used in traditional and folk medicine. Its flowers, bark and leaves arean important medicinal raw because it possesses large amounts of the tanic substances, carotenoids,isovalerianic acid, saponines, and glycosides. The seeds contain up to 21 % of fatty oil (Bock et al.,1978). Fruit of this species accumulate significant amounts of biologically active substances. The18
fruit of V. opulus has different amounts of ascorbic acid, pectin, carotenoids, flavonols,tocopherols, anthocyanins, different polysaccharides (Евтухова et al., 2000; Евтухова et al., 2002;Лобанова et al., 1999; Оводова et al., 2000; Velioglu et al., 2006; Jordheim et al., 2007).The collection of V. opulus accessions at Kaunas Botanical Garden was started in 1998. Theevaluation of collected samples was done during 1999 – 2008. The most comprehensive researchwork on Viburnum sp. was accomplished hitherto in Russia and Ukraine. Different cultivars wereadded to the Russian official register and recommended for growing (Куденков М. andЧурканенко Н., 1998). The Ukrainian selections have small bushes, dark red fruits with a weakerbitter quality and a yield of 8 – 9 t ha -1 (Panteev et al., 1995).The investigation of productivity, fruit properties and biochemical components of V. opulusaccessions and the selection of the most valuable clones and cultivars makes it possibility to usethem for breeding.The aim of present study was to investigate the variation of yield, fruit properties and biochemicalcomponents of V. opulus accessions and select the most valuable clones and cultivars forpreservation and breeding in Lithuania.Materials and methodsThe European cranberrybush cultivars of Russian origin, the clones P1 (of Lithuanian origin) andP2 (of Ukrainian origin) were selected for evaluation. All accessions are proving easy to grow andexceptionally winter hardy. Fruit production began within two years after planting.The yield and fruit properties (yield per bush, number of fruit in a cluster and mean weight of afruit) were studied. The results were statistically analyzed and regression (R 2 ) and variation (CV)coefficients were calculated using STAT for Excel.For the average fruit weight 100 fruits were collected and examined. 20 racemes were examined toevaluate fruit number per raceme and 10 bushes were examined to evaluate the yield per bush..The investigations of the biochemical composition of the fruit were conducted in their maturestage. The amount of ascorbic acid was ascertained by the Tilmans reaction. It was titrated by asolution of 2.6-dichlorphenolindophenol sodium salt. The pigments were extracted with 95 % (v/v)grade ethanol acidified with 0.1 M HCl with the purpose to assay the total amount of anthocyanins.The total amount of anthocyanins was expressed by cyanidin 3-rutinoside. The amount of flavonolswas expressed by rutin and determined spectrophotomerically at a wavelength of 440 nm. Themethod of benzoic acid determination was based on steam distillation. The spectrophotometricanalysis was carried out by a reaction of benzoic acid with hydroxylamine-HCl and peroxide at awavelength of 315 nm (Ермаков А. and Арасимович В., 1987; Helrich, 1990).Results and DiscussionThe investigation showed that accessions differed in their yield per bush, the number of fruit in acluster and the mean weight of the fruit as well. Reliable differences between the cultivar‘Leningradskaja Otbornaja’ and other accessions were determined. A constant large average yieldwas typical of the cultivars ‘Leningradskaja Otbornaja’, P3 and ‘Zarnica’ 8.5 – 6.2 kg per bush.The cultivars ‘Zarnica-2’, ‘Souzga’, and ‘Šukšinskaja’ had the lowest yields 2.6 – 3.2 kg per bush(Table 1).The accessions of V. opulus were distinguished by significant differences in the size of a raceme.The number of fruit in a raceme varied from 28.1±5.83 (‘Upninkai’) to 51.0±6.1 (cv. ‘KrasnajaGrozd’).The cultivars ‘Upninkai’ and ‘Leningradskaja Otbornaja’ produced the largest fruit (0.74±0.09 gand 0.66±0.05 g, respectively). The cultivar ‘Šukšinskaja’ and P3 were typical of the smallest fruit,0.45±0.04 g and 0.47±0.04 g, respectively. Tge length of a cluster stalk varied from 2.9±0.10(‘Leningradskaja Otbornaja’) to 4.6±0.21 cm (P3).Statistically reliable differences were determined between the mean weight of a fruit. Estimates ofvariability indicated that the most stable trait was the mean weight of a fruit. The coefficient ofvariation did not exceed 20 %, except in the cv. ‘Krasnaja Grozd’ (22.7 %). The stability of fruitnumber in a cluster differed subject to the accession. The variation of fruit number in a cluster ofaccessions P1 and ‘Leningradskaja Otbornaja’ was low (CV < 10 %). The medium coefficient ofvariation (10 % < CV < 20 %) was determined for the clone P2 and the cultivars ‘Krasnaja Grozd’,19
- Page 3 and 4: Conference Organizing CommitteeChai
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The contribution of V. macrocarpon
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11. Kong J. M., Chia L. S., Goh N.K
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isothermically at 70°C for 5 min,
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IN VITRO PROPAGATION OF SEVERAL VAC
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16BM ean N o. of shoots/explant1412
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Figure 2. Axillary shoot regenerati
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evaluate the blueberries supply wit
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espectively). It should be stressed
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lueberry appear to play a conclusiv
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15. Reimann C., Kollen F., Frengsta
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each type, and for comparison sampl
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the mean. Kisgyır 1 sample has the
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13. Porpáczy A. (1999) A húsos so
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was medium (0.014 - 0.017 g kg -1 s
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‘Salaspils Ražīgā’. Vigorous
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KopsavilkumsEiropas melleĦu (Vacci
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Figure 2. Chemometric PCA of 32 blu
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References1. Baloga D.W., Vorsa N.,
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obtained from fruits of black choke
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In our opinion, the best estimate a
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cuttings also varies markedly with
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shoots shorter than 10 mm were not
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14. Ostrolucka M.G., Gajdosova A, L
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„Metos RG-350” (http://www.meto
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500480Phenols,mg 100g -146044042040
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SHORT INFORMATION ABOUT THE HISTORY
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Evaluation of cultivars. After the
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the number of pistils (female clone
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Table 2. Number of flowers per harv
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ResultsFirst time upright dieback i
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grew rapidly on PDA at 20 - 24 o C.
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Figure 9. Conidia of Physalospora v
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References1. CABI, EPPO, (1997) Dia
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Results und DiscussionBerries were
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In literature Caruso eds. and Гop
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the total area under a cranberry ma
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Skilled works on development of the
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Tika atrastas dažas būtiskas ats
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appears to maintain a quite low lev
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8. Garkava - Gustavson L.,Persson H