Figure 1. Conidia of Fusicoccum putrefaciens on PDA (400x).According to symptoms upright dieback, blossom and ovary blight and end rot were caused byFusicoccum putrefaciens Shear. The teleomorph stage caused by Godronia cassandrae Peck f.vaccinii Groves was not found in Latvia. The causal agent of the disease was identified based onsymptoms and the morphological characteristics described by Гopлeнкo et al., 1996 and Caruso F.L., 1995.Phomopsis vaccinii from upright dieback, blossom and ovary blight and viscid rot samples wasdetected. Uprights, blossoms and ovaries turned brown and died, but viscid rot was off-color,slightly mottled or yellowish brown and firm but wet inside with a viscous, sticky substance.Viscid rot was common in the field and during the first months of storage. Colonies on the PDAgrew up to 15 mm per day; white, circular, and near to centre dark rings were produced. The aerialmycelium was not compact, was grayish white, and toward the centre a wall was produced. Thepycnidia mostly set on the wall. They were 1 – 4 mm in diameter, partly embedded, leathery, palegrey and then turned black (Figure 2). From maturity pycnidia emitted a yellow creamy spore massin the moisture camera on uprights dieback and the pure culture. P. vaccinii had two types ofspores (Figure 3). Alfa conidia were hyaline, one-celled, ellipsoid, with two oil globules at bothends and measured 7.8 x 3.1µm (4.3 – 9.8 x 2.0 – 4.4 µm). Beta conidia were unicellular, hyaline,filiform – hook-shaped at the end, and measured 18.6 x 0.8 µm (13.4 – 22.1 x 0.3 – 1.2 µm).Fig. 2. Pycnidia of P.vaccinii on PDA(10 x).Fig. 3. α and β conidia of P. vaccinii onPDA (400 x).According to the symptoms of cranberry disease and fungus anamorph morphological peculiaritiesin the moisture camera and pure culture, upright dieback, blossom and ovary blight and viscid rotwere caused by Phomopsis vaccinii Shear in Shear, N. Stevens, & H. Bain. The teleomorph stage,which is caused by Diaporthe vaccinii Shear in Shear, N. Stevens, & H. Bain., was not detected inLatvia. The causal agent of the disease was identified based on symptoms and morphologicalcharacteristics as described by EPPO, 1997 and Kačergius et al., 2004.Discosia artocreas from upright dieback, blossom blight and berry rot was detected only in fewsamples. Young uprights were bronze, brown with end of the top sloped and the uprights of lastyear were dark brown. Blossoms damaged by disease were brown, but discosia fruit rot was withyellowish brown spots. Discosia artocreas mostly was detected from upright dieback. The fungus128
grew rapidly on PDA at 20 – 24 o C. Aerial mycelium was low, compact, leathery and pale gray.Mycelium produced paler irregular rings. Colonies were dark pale. In the moisture camera onuprights dieback and pure culture appeared pyriform, dark grey pycnidia. From maturity pycnidiaemitted a yellowish white, creamy spore mass. Separately conidia were hyaline or pale grey,oblong, measured 3.2 x 14.2 µm (2-4 x 12-17 µm); 2 – 3 septates, end of tops had two longappendages (Figure 4). In the pure culture septates and appendages of conidia hardly wereobserved.Figure 4. Conidia of Discosia artocreas in moisture camera and PDA (400 x).According to the symptoms of cranberry disease and fungus morphological peculiarities inmoisture camera and pure culture was caused by Discosia artocreas (Tode) Fr. The teleomorphstage of the fungus caused by Gnomonia setae was not detected. The causal agent of the diseasewas identified based on symptoms and morphological characteristics as described by Гopлeнкo etal., 1996.Pestalotia vaccinii caused upright dieback, blossom blight and pestalotia fruit rot in Latvia.Uprights were bronze, with end of the top sloped. They were spread only in several cranberryplantations and the incidence level was not high. In storage on some berries appeared yellowbrown,circular, slightly sunken rot spots with darker, concentric rings. Fungus grew rapidly on thePDA, at 20 – 24 o C. The aerial mycelium was fluffy, at the centre lemon-white, up to marginswhite appeared. Colonies were lemon-yellow. In the culture through mycelium appeared black,watery, and spore mass was scroll-shaped (Figure 5). In the moisture camera on uprights diebackacervuli matured also. The conidia were elongated fusoid, straight or slightly incurved andmeasured 5.8 x 27.5 (4.7 - 6.8 x 22 - 32 µm). The conidia had five-cells, the apical and basal cellswere hyaline, but inside three cells were green-brown (Figure 6). The conidia had appendages atboth ends. The end of the basal cell had on average a 13.7 µm (9.5 – 18 µm) long appendage, butthe end of the apical cell had 3 – 4 on average 23.9 µm (16 – 33 µm) long moustached appendages.In the culture appeared hyaline, ellipsoid, curved microconidia, in diameter 2.0 x 6.3 µm (1.3 - 2.7x 4.5 – 7.8 µm).Figure 5. Spore mass of P. vaccinii on PDA.Figure 6. Conidia of P. vaccinii on PDA(400x).According to the symptoms of cranberry disease and fungus morphological peculiarities in themoisture camera and the pure culture was caused by Pestalotia vaccinii (Shear) Guba. The causal129
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Conference Organizing CommitteeChai
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15 Pormale J., Osvalde A. and Nolle
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were established in 1985. Nowadays,
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10,1-15 ha7%15,1-20 ha7%< 20,1 ha0%
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In less than half the surveyed farm
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economical and biochemical characte
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investigated European cranberry acc
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fruit of V. opulus has different am
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As several authors have stated (Koz
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KopsavilkumsVaccinium ăints kultū
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maintained in a mist chamber with v
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period and produce vigorous vegetat
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38. Marcotrigiano M. and McGlew S.P
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of changes in the typological struc
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fall from 2 to 3 and that for heath
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HIGHBUSH BLUEBERRY BREEDINGAUGSTKR
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Southern and Intermediate highbush
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and anatomically they belong to fal
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The levels of flavonols are more co
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21. Polashock J.J., Griesbach R.J.,
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Figure 1. A general scheme of the N
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5. Åkerström A., Forsum Å., Rump
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species and studying the efficiency
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Thus, it has been determined that t
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CHEMICAL COMPOSITION OF HIGHBUSH BL
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lueberry cultivars were collected f
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Ascorbic acid, mg 100ḡ 112108642a
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6. Saftner R., Polashock J., Ehlenf
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Materials and methodsThe experiment
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The titrable acids content of the e
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There was a significant correlation
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Nichenametla et al., 2006), human n
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The contribution of V. macrocarpon
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11. Kong J. M., Chia L. S., Goh N.K
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isothermically at 70°C for 5 min,
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IN VITRO PROPAGATION OF SEVERAL VAC
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- Page 79 and 80: Figure 2. Axillary shoot regenerati
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- Page 89 and 90: each type, and for comparison sampl
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- Page 93 and 94: 13. Porpáczy A. (1999) A húsos so
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- Page 101 and 102: Figure 2. Chemometric PCA of 32 blu
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- Page 119 and 120: SHORT INFORMATION ABOUT THE HISTORY
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- Page 127: ResultsFirst time upright dieback i
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- Page 133 and 134: References1. CABI, EPPO, (1997) Dia
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