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Honeybee Production and Marketing Systems, Constraints - IPMS ...

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Appendix 7.5 Method of Chemical Solution Preparation0.025M sulpheric acid solution: Pipate 0.68ml of concentrated sulpheric acid <strong>and</strong> diluteit to 500ml with distilled water0.05M NaOH solution: Accurately weigh 1gm of NaOH <strong>and</strong> dilute it to 500ml withdistilled waterCarrez solution I: dissolve 15 g of potassium hexacyanoferrate (II), K4Fe (CN) 6.3H2Oin water <strong>and</strong> make up to 100 ml.Carrez solution II: dilute 30 g of zinc acetate, Zn (CH 3 .COO)2.2H 2 O <strong>and</strong> make up to100 ml. Sodium bisulphite solution 0.20 g/100 g: dissolve 0.20 g of solid sodiumhydrogen sulphite NaHSO3, (metabisulphite, Na2S2O5), in water <strong>and</strong> dilute to 100 ml.Prepare fresh daily.Sodium chloride solution: dissolve 2.9 g of sodium chloride in water <strong>and</strong> dilute to 100ml.Acetate buffer solution (pH 5.3): dissolve 43.5g of sodium acetate (CH3.COONa.3H2O) in water, adjust the pH of the solution to 5.3 with about 5 ml of glacial acetic acid<strong>and</strong> dilute to 250 ml with water.Starch solution:Determination of starch dry weight: Spread approximately 2 g of air dry soluble starch ina thin layer over the bottom of a weighing bottle (diameter 5 cm, height 3 cm) with a lid.Weigh accurately (± 0.1 mg) <strong>and</strong> dry for 90 minutes at 130°C.Allow the closed weighing bottle to cool for about 1 hour in a desiccator <strong>and</strong> re-weighaccurately.Soxhlet’s modification of Fehling’s solution:Solution A: dissolve 69.28 g of Copper sulphate pentahydrate (CuSO 4 .5H 2 O in water,MW = 249.71) <strong>and</strong> make up to 1000 ml. Keep one day before titration.96

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