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Honeybee Production and Marketing Systems, Constraints - IPMS ...

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homogenized honey samples. The refractive index after 2 minutes was read the correspondingmoisture content from the table. Water contents of sample were measured twice <strong>and</strong> the averagevalue was recorded. Finally the prism was cleaned after use.The table is derived from a formula developed by Wedmore, (1955) from the data of Chataway,(1932) <strong>and</strong> others:-W=1.73190− log( R.I − 1)0.002243WhereW is the water content in g per 100 g honey <strong>and</strong> R.I. is the refractive index3.2.4.2 Mineral (ash) ContentAsh content was determined after the sample was burnt in an electric muffle furnace (LentonThermal Designs, Engl<strong>and</strong>). First the ash dish was cleaned <strong>and</strong> heated in the electrical furnace atashing temperature, subsequently cooled in a dessicator to room temperature <strong>and</strong> weighed to0.001g (M 2 ). Then, 5 gram of honey sample was weighed to the nearest 0. 001g (M 0 ) <strong>and</strong> put inthe prepared ash dish <strong>and</strong> two drops of olive oil was added to prevent frothing. Then, water wasremoved <strong>and</strong> commences ashing without loss (by foaming <strong>and</strong> overflowing) at a low heat risingto 350 - 400 0 C by using electrical device. Hot plate was used to char the sample before insertinginto the furnace. After the preliminary ashing with hot plate, the dish was placed in the preheatedmuffle furnace (at 550 0 C) <strong>and</strong> heated for 1 hour. The ash dish was cooled in the desiccator <strong>and</strong>weighted. The ashing procedure was continued until constant weight is reached (M 1 ). Percent ashin g/100g honey is calculated using the following formula:Calculation <strong>and</strong> expression of resultM 1 - M 2Ash % = ----------- x 100M 0Where M 0 = weight of honey taken, M 1 = weight of dish + ash <strong>and</strong>, M 2 = weight of dish.29

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