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Cancer biologyB-17-13Syntenin positively regulates transforming growth factor-β1-mediatedEMT and Smad2/3 activationCheol Hwangbo, Nara Tae, Jaekyung Kim and Jeong-Hyung LeeDepartment of Biochemistry, College of Natural Sciences, Kangwon National University,Chuncheon, Gangwon-Do 200-701, KoreaSyntenin, a tandem PDZ-domain containing scaffold protein, functions as a positiveregulator of cancer cell progression in several human cancers. In the present study, weinvestigated the role of syntenin in TGF-β1-induced EMT and Smad activation in A549cells. Knockdown of syntenin resulted in suppression of TGF-β1-induced cell morphologicchanges and migration, and abolished TGF-β1-mediated repression of E-cadherinexpression, and up-regulation of vimentin and Snail expression. In contrast,overexpression of syntenin into A549 cells facilitated down-regulation of TGF-β1-inducedE-cadherin, and up-regulation of vimentin and Snail. In addition, knockdown of synteninblocked TGF-β1-mediated transcriptional responses, and phosphorylation and nucleartranslocation of Smad2/3; however, overexpression of syntenin dramatically increasedTGF-β1-mediated transcriptional responses, and phosphorylation of Smad2 and Smad3.Knockdown of syntenin also blocked TGF-β1-mediated activation of Smad2 and Smad3,as well as cell migration in different types of cells. These findings demonstrate for the firsttime that syntenin may act as a critical modulator of TGF-β1-activated Smad signaling.B-17-16Gene expression profiling of MCF 10A series of Ha-Ras transformedhuman breast epithelial cellsSihyung Yang¹, April E. Cho²and So Hee Kim³¹Department of Pathology and Laboratory Medicine, School of Medicine, University of NorthCarolina at Chapel Hill, NC, USA, ²Global Ecomonics, Sung Kyun Kwan University, Seoul 110-745, ³Department of Pharmacology, College of Dentistry, Gangneung-Wonju National University,Gangneung 210-702, KoreaMCF10A series (10A, 10AT, 10ATG3B, 10CA1h and 10CA1a) of breast epithelial cellswere examine for gene expression involved in epithelial-mesenchymal transition (EMT)process. This model represents a unique system for examining early and progressivealterations that occur in cells. Mmicroarray analysis showed more than 720 genes arehighly expressed (>2 fold, P