11:10-12:00, Rm 103

MicrobiologyG-17-25Discovery of chemical compounds that inhibit SARS-CoV helicaseactivity with high-throughput screeningMi-Sun Yu, Jin-Moo Lee and Yong-Joo JeongDepartment of Bio and Nano chemistry, Kookmin University, Seoul 136-702, KoreaSevere acute respiratory syndrome(SARS) is a respiratory disease which is occured bythe SARS coronavirus(SARS-CoV). SARS has a case-fatality rate of 9.6%.(According tothe WHO, 21 April 2004) The helicase protein from SARS-CoV is a requisite for virusreplication. We study the SARS-CoV helicase as a target for anti-SARS therapy. Wehave expressed and purified this protein using Ni-chelated affinity chromatography andSephadex G-100 gel-filtration column chromatography as an N-terminal hexahistidine tagand size exclusion. Assay of high-throughput screening(HTS) is used to find out inhibitorsof SARS-CoV helicase. HTS assay is carried out 2 parts, first part is ATP hydrolysisactivity test and second part is FRET-based double stranded DNA unwinding activity test.The compounds YC99 and YC102 had IC50 values of 9.5030 ±0.2348μM and 3.9072 ±0.0440μM for ATP hydrolysis test, respectively. The compound YC102 had an IC50 valueof 10.9761 ± 0.2949μM for double stranded DNA unwinding test. We expect that the YCcompounds will useful for anti-SARS therapy.G-17-26Classification of the sublineages of mycobacterium tuberculosis beijingfamily isolated in the republic of koreaAhreum Baek , Hyeeyn Bang , Younim Choi , Hyeyoung LeeDepartment of Biomedical Laboratory Science, College of Health Science, Yonsei University,Wonju, KoreaThe Beijing family of Mycobacterium tuberculosis (Mtb) is one of the most successfulclades in the present worldwide tuberculosis (TB). At present, RFLP is the mostdiscriminating test of all standard DNA finger printing. DNA fingerprinting of Beijing familyof Mtb is very important to control the TB. However, RFLP is time consuming, expensiveand requires skilled experts. Therefore, limited laboratories can perform and inappropriatefor clinical setting. In this study, we have developed PCR-based method for genotyping ofBeijing family using specific regions of protein coding sequences. We found unique bandwhich may provide more useful information of Beijing sublineages than RFLP method.These findings indicate that it is possible to classify of the Mtb Beijing sublineages in theRepublic of Korea. To establish the usefulness of this newly developed method, moretests need to be performed with more clinical samples.242 Korean Society for Biochemistry and Molecular Biology