11:10-12:00, Rm 103

GE Healthcare Ltd.High content analysis as a tool for predictive toxicology in human celllinesJ. Michael HoneysettMarket Development Leader, Global, GE Healthcare, Life Sciences Piscataway, NJ 08854, USAOne of the key challenges in drug discovery is the rapid identification and characterizationof compounds that induce cytotoxicity. In today’s drug screening lab, the tools andprocesses that are employed for the identification of cytotoxic compounds are oftenpainstaking and time consuming, prolonging time-to-market. Further, the industry is oftenburdened with a number of drug candidates that slip through the pipeline into veryexpensive animal studies and, in some cases, into clinical trials. In this talk, a novel HighContent Screening technology will be presented that shows promise in the earlyidentification of cytotoxic compounds, on many different levels. The technology provides afully automated multi-parametric method that simultaneously monitors changes in cellmorphology, cell cycle, and cell proliferation. Further, subtle changes in nuclearmorphology, mitochondrial status and membrane leakage can be monitored with thetechnology, all simultaneously, along with changes related to the formation of micro-nucleiand early DNA strand breakage. As a developmental tool, the technology shows greatpromise in that it provides detailed information on cytotoxicity early in decision makingprocess, thereby improving prod.NIKON INSTRUMENTS KOREA CO., LTD NIKSuper-resolution microscopy: N-SIM and N-STORMSunghoe ChangDepartment of Physiology, Seoul National University College of Medicine, Seoul 110-799, KoreaResolution of conventional optical microscopes even with the highest numerical apertureoptics are limited by diffraction to approximately 200 nm. Recent developments of superresolutionfluorescence microscopy techniques, however, allow us to observe threedimensionalbiological structures, to measure interactions by multicolor colocalization, andto record dynamic processes in living cells at the nanometer scale, which were notresolvable in conventional fluorescence microscopy. Nikon’s new Super Resolutionmicroscope N-SIM/N-STORM enables elucidation of structure and function of thenanoscopic machinery within living cells. Using Structured Illumination, the N-SIM canachieve image resolution of 85 nm with temporal resolution of up to 0.6 sec./frame,enabling super-resolution time-lapse imaging capture of dynamic molecular interactions inliving cells. N-STORM realizes an incredible image resolution with lateral resolution toapproximately 20 nm and axial resolution to approximately 50 nm, which is 10 times ormore than that of conventional optical microscopes. Utilizing N-STORM, it will be capableof multi-spectral two-dimensional and three-dimensional nanoscopy, extending the role ofthe optical microscope to near molecular level resolution.Dong-il SHIMADZU Corp.Introduction of active-gassing system for cell cultureTakeshi SuzukiTAITEC Corporation, 2693-1 Nishikata, Koshigaya-shi, Saitama-ken, 343-0822, JapanCell culture system becomes more important technology not only in basic science butalso bioprocess of bio-medicine in these days. In the bio-process, high-cell density isimportant of manufacturing efficiency. However, conventional methods have somedisadvantages in large scale cell culture in the point of control of dissolved oxygen (DO),pH and prevention of contamination. We TAITEC, Japanese specialty company for cellculture, propose active-gassing technology for high-density culture. Active-gassingsystem maintains the optimum medium-condition by purging sterilized gas into chambersdirectly. Our gassing system is available both suspension cell and static cell culture. Wesupport research scale (Erlenmeyer flask, multi-well plate or TC flask) to pilot scale forproduction (culture-bag or multilayer tray). Monitoring of DO and pH is required in scaleupof culture. TAITEC is developing non-invasive optical sensors for process control andvalidation without contamination risk. Our products will contribute the advance in bioprocessbased on mammalian cell culture system. In this presentation we would like toshare the advantages of active-gassing for production of biopharmaceuticals withintroduction of technical data.Bio-Medical Science Co., LtdPersonal genomics study using the Illumina MiSeq systemKyunga KimBio-Medical Science Co., LtdIn past two years, Genomics research has been revolutionized by the NGS technology.The MiSeq personal sequencing system delivers the fastest time to answer, arevolutionary workflow, and the widest breadth of sequencing applications, all in acompact and economical instrument. For results in hours, not days, MiSeq uses TruSeq,Illumina's reversible terminator-based sequencing by synthesis chemistry in combinationwith innovative engineering. Perform standard experiments such as amplicon sequencing,clone checking and small genome sequencing with enhanced performance, more quickly,and cost-effectively compared to capillary electrophoresis (CE) sequencing. Gain accessto powerful next-generation sequencing applications such as highly multiplexed PCRamplicon sequencing, targeted resequencing, ChIP-Seq, small RNA sequencing, andmore. For current next-generation sequencing users, complete smaller projects in afraction of time and cost using the MiSeq systemWe will carefully review our customersuccess in all applications.Hucom System Co., Ltd.An introduction of Dual Polarisation Interferometry technologyVanilla ChenSouth East Asian Distribution Manager, Biolin Scientific AB, China Representative OfficeRm.1603, 1101 Pu Dong Road(s) Shanghai 200120, ChinaDual polarisation interferometry (DPI) is a method with considerable potential in assessingsmall difference in the structure of proteins bound to a surface. The technique utilizes twolight beams passing through a waveguide. After passing through the waveguide, the twolight beams produce an interference pattern. The presence of a protein or anothermolecule on the surface causes altered polarisation of one of the light beams, changingthe interference pattern generated by the instrument. Changes in the measured refractiveindex are indicative of thickness changes at the waveguide surface. From this information,changes in density, mass of the protein complex and volume can be determined. DPIextends the typical dynamic measurements of conventional biosensors by including anadditional quantitative, sub molecular, conformational measurement. It delivers a uniqueperspective on biochemistry, linking conformational changes to biochemical activity athigh resolution.Young Wha Scientific Co., LTD.Lonza shifts nucleofection to a new dimension - introduction of the 4Dnucleofector and HT nucleofector systemsScott D'AndreaLonza Walkersville,Inc. 8830 Biggs Ford Road Walkersville, Maryland 21793, USANucleofector Technology is based on two unique system components. The Nucleofecto System delivers optimized electrical parameters and Nucleofector Kits, which containcell-specific Nucleofector Solutions. As part of Nucleofector Technology, Lonzaprovides cell-type specific Optimized Protocols for many different cell lines and primarycells; including protocols for neurons, blood cells, stem cells, endothelial, epithelial, andcancer cells. With over 160 protocols optimized by Lonza’s R&D team for cell lines andprimary cells and more than 680 entries in the online cell data base, Nucleofector Technology is the transfection method-of-choice for most any difficult-to-transfect celltype. Lonza's novel modular Nucleofection Platform allows transfection of cells in variousformats and throughputs using the same Nucleofection Conditions. is also introducingthe HT Nucleofector System designed for high throughput screening to be released fall2010. The HT Nucleofector System will provide researchers with the ability to transfect384 samples in less than one minute, employing the same technology, protocols andreagents utilized with the 4D Nucleofection System.94 Korean Society for Biochemistry and Molecular Biology