11:10-12:00, Rm 103

Cancer biologyB-17-183Clinical validation of colorectal cancer biomarkers identified frombioinformatics analysis of public expression dataYeonjoo Jung¹ , ², Sanghyuk Lee¹ , ² , ³, Soon-Nam Kim¹ , ², Yeonhwa Jung¹ , ², Hee-Young Lee¹ , ², Juhee Keum¹ , ², Wan Kyu Kim¹ , ², Ho-Kyung Chun⁴, Woo Yong Lee⁴,Jaesang Kim¹ , ²¹Ewha Womans University, Ewha Research Center for Systems Biology, Seoul 120-750, ²EwhaWomans University, Division of Life and Pharmaceutical Sciences and the Center for CellSignaling & Drug Discovery Research, Seoul 120-750, ³Korean Bioinformation Center, KOBIC,Daejeon 305-806, and ⁴Samsung Medical Center, Sungkyunkwan University School of Medicine,Department of Surgery, Seoul 135-710, KoreaIdentification of novel biomarkers of cancer is important for improved diagnosis,prognosis, and therapeutic intervention. This study aimed to identify marker genes ofcolorectal cancer (CRC) by combining bioinformatics analysis of gene expression dataand validation experiments using patient samples and to examine the potentialconnection between validated markers and the established oncogenes such as c-Mycand K-ras. Publicly available data from GenBank and Oncomine were meta-analyzedleading to 34 candidate marker genes of CRC. Multiple case-matched normal and tumortissues were examined by RT-PCR for differential expression, and 9 genes werevalidated as CRC biomarkers. Statistical analyses for correlation with major clinicalparameters were carried out, and RNA interference was used to examine connection withmajor oncogenes. We show with high confidence that 9 of the 34 candidate genes areexpressed at significantly elevated levels in CRC tissues compared to normal tissues. Wehave identified multiple novel biomarkers of CRC, and further analyses of their functionand connection to signaling pathways may reveal potential value of these biomarkers indiagnosis, prognosis, and treatment of CRC.198 Korean Society for Biochemistry and Molecular Biology