11:10-12:00, Rm 103

Chemical biology and drug discovery Anal S),without hemolytic effects. Moreover, the membrane studies, diSC₃5 staining, rhodamineleakage from a single giant unilamellar vesicle (GUV), and FITC-dextran leakage assaydemonstrated that the analogs as well as Rev-NIS acted on the bacterial membranes andpotently made pores, with the hydrodynamic radius between 1.4 nm and 2.3 nm.Especially, Anal R made pores with the radius between 2.3 nm and 3.3 nm. These resultsalso corresponded to the result of antibacterial susceptibility testing. In summary, thisstudy suggests that the two arginine residues are more influential than the hydrophobicityor the helicity, regarding the molecular activity of the peptide.D-17-24Activation of AMP-activated protein kinase on human gastric cancercells by apoptosis induced by corosolic acid isolated from weigelasubsessilisEun Young Cha, Myung Sun Lee, Chang Min Lee, Phuong Thien Thuong, KiHwanBae,In Sang Song, Seung Moo Noh and Ji Young SulRegional Cancer Institute, Chungnam National University Hospital, Daejeon, Korea, Departmentof Surgery, Chungnam National University Hospital, Daejeon, Korea, Vietnam National Instituteof Medicinal Materials, Hanoi, Vietnam College of Pharmacy, Chungnam National University,Daejeon, Korea, Department of Surgery and Research Institute for Medicinal Sciences, ChungnamNational University School of Medicine, Daejeon, KoreaCorosolic acid is one of the triterpenoids present in the leaves of Weigela subsessilis. Theantidiabetic activity of corosolic acid has been reported previously, but to date, theanticancer effects on gastric cancer have been poorly studied. In this study, corosolic acidshowed growth inhibition on SNU 601 human gastric cancer cells, with an IC50 value of16.9 ± 2.9 μM. Corosolic acid also triggered the activation of caspase-3 and poly(ADPribose) polymerase, while it was recovered by Z-VAD-FMK. Moreover, the cellgrowth/apoptosis activities of corosolic acid were regulated by the AMP-activated proteinkinase-mammalian target of rapamycin (AMPKmTOR) signals. These results showedthat corosolic acid-mediated AMPK activation leads to inhibition of mTOR, thus providinga possible mechanism of action of corosolic acid in the inhibition of cancer cell growthand the induction of apoptosis.Poster Session2011년도 생화학분자생물학회 연례국제학술대회221