Chromosome segregation errors: a double-edged sword - TI Pharma

5
A
B
DIC
Propidium
Iodide
C
D
100
PI positive cells (%)
PI-positiive cells (%)
70
60
50
40
30
20
10
0
80
70
60
50
40
30
20
10
U2OS-TetRMps1
0
n=1058
n=1180
n=548
n=459
n=432
n=455
n=285
n=153
0 2 5 10 0 2 5 10 nM taxol
n=919
LS174T-TetR Mps1
0:00 04:00 17:00 17:40 19:30 48:30 49:20
-
n=761
live cell analysis
n=449
n=748
+
n=565
n=87
doxycycline
0 1 2 0 1 2 nM taxol
- +
doxycycline
- doxy + doxy - doxy + 3nM tax + doxy + 3nM tax
U2OS-TetR Mps1
HeLa-TetR BubR1
Figure S2.
A) Representative DIC images of U2OS-TetRMps1 cells undergoing cell death after doxycycline and 5nM taxol treatment. Propidium
Iodide was added to the culture medium to determine uptake by dying cells. B+C) Quantification of time-lapse analysis performed
as in (A) of cell death induction in U2OS-TetRMps1 and Hela-TetRBubR1 cells treated with or without doxycycline and indicated taxol
concentrations on day 4, 5 and 6. DIC and PI images were acquired every 15 minutes. The percentage of cell death was measured as
the fraction of PI positive cells over the total amount of cells (n) at the end of the experiment. D) Representative images of LS174T-
TetRMps1 colonies 11 days after indicated treatments.