Chromosome segregation errors: a double-edged sword - TI Pharma

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(LabTekII) and imaged after addition of nocodazole in a heated chamber (37°C and 5% CO2) using a
×20/0.5NA UPLFLN objective on a Olympus IX-81 microscope, controlled by Cell-M software (Olympus).
Images were acquired using a Hamamatsu ORCA-ER camera and processed using Cell-M software.
Antibodies
Rabbit-Anti-phospho-Threonine 676 (Msp1) was described before 172 . Rabbit-anti-ESK (sc541) was
from Santa Cruz. Mouse anti-MPM2 was from Millipore, Human ACA (Crest) was from Fitzgerald
Industry and Mouse anti-GFP from Roche. Anti-human Alexafluor647 and anti-rabbit Alexafluor568
were from DAKO, anti-mouse cy-5 from Jackson.
Acknowledgements
We want to thank Patrick Derksen, Jos Jonkers, Alain de Bruin, Tobias Dansen and Miranda van
Amersfoort for valuable input. We are also grateful to Hans Clevers for providing ES cell targeting
facilities and Stieneke van den Brink for help with the ES cell recombination. We thank Livio Kleij
for assistance with the microscopes, the personnel of the animal facilities of the Gemeenschappelijk
Dieren Laboratorium (GDL) in Utrecht for animal husbandry and Rika Kramer and Marco Hoekman
from the Transgenesis Facility at the GDL for performing the blastocyst injections and generation of
the CiMKi chimeric mice. We also want to thank all members of the Medema, Kops and Lens labs
for their input during lab meetings. This work was supported by the Netherlands Organisation for
Scientific Research (NWO) (R.H.M. and A.J.: ZonMw 918.46.616; G.J.P.L.K.: VIDI-91776336), TI Pharma
(R.H.M and A.J.: T3-105) and the ERC (G.J.P.L.K. and N.J.: ERC-StG KINSIGN). R.H.M. was additionally
funded by the Netherlands Genomic Initiative of NOW and N.J. by the Dutch Cancer foundation (KWF):
UU 2012-5321.
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