Chromosome segregation errors: a double-edged sword - TI Pharma

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Chromosome segregation errors: a double-edged sword Aniek Janssen
ISBN: 9789088915383 Printed by: Proefschriftmaken.nl || Uitgeverij BOXPress Published by: Uitgeverij BOXPress, ‘s-Hertogenbosch Cover: An immunofluorescence image is shown of a cancer cell that missegregates its chromosomes (blue) in telophase upon treatment with an Mps1 inhibitor
Page 4 and 5: Chromosome segregation errors: a do
Page 6: Get up, stand up, stand up for your
Page 10 and 11: Chapter 1 General Introduction Anie
Page 12 and 13: cytoplasms and pinches off the memb
Page 14 and 15: which sister-chromatids are not pro
Page 16 and 17: Non-MCC members, but important mito
Page 18 and 19: completely separated. Following cle
Page 20 and 21: Two striking features of cancer cel
Page 22 and 23: predisposition at a very young age,
Page 24 and 25: that aberrant spindle formation inc
Page 26 and 27: (Fig.7C). As discussed in section 4
Page 28 and 29: Thesis outline Chromosome segregati
Page 30: 286,395,407,408 Increase (thymus) I
Page 33 and 34: 2 Abstract Various types of chromos
Page 35 and 36: 2 34 A C D E % of cells Control 100
Page 37 and 38: 2 assess whether cytokinesis induce
Page 39 and 40: 2 A Asynchronous Monastrol release
Page 41 and 42: 2 for 14 hours (unless indicated ot
Page 43 and 44: 2 Immuno Blotting Cells were lysed
Page 45 and 46: 2 Supplemental figures 44 A B C % o
Page 47 and 48: 2 A MCF7 B C 53BP1 foci/cell 46 % o
Page 49 and 50: 2 A siLuc siATM C 48 p-S1981 ATM me
Page 51 and 52: 2 50 A B C % of EdU positive cells
Page 54 and 55:
Chapter 3 Studying Chromosomal inst
Page 56 and 57:
Introduction Aneuploidy is a common
Page 58 and 59:
activity by ~80% 170-172 . With the
Page 60 and 61:
A WT/CiMKi T649A Embryo’s: Figure
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control treated MEFs (unpaired t te
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Materials and Methods Cloning targe
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- Loop out BamHI site with site-dir
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Primers: pGH_pA_FW#2: TCTATGGCTTCTG
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was prepared using standard procedu
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71 Cre-inducible Mps1 knock-in mous
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4 Abstract Most of the current drug
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4 Two other interesting mitotic tar
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4 inhibition of the anti-apoptotic
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4 cluster their centrosomes. This
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4 of checkpoint function can be ach
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4 4.5 Disadvantages of exploiting m
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Chapter 5 Elevating the frequency o
Page 90 and 91:
Introduction Error-free chromosome
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Partial depletion of Mps1 or BubR1
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death is not induced in the short t
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had survived growth for 7 days in t
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The following antibodies have been
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Supplemental data Supplemental figu
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A BubR1 α-tubulin Hela-TetRBubR1 -
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- doxycycline + doxycycline - doxyc
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A B C percentage of cells % PI posi
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Abstract One of the most common hal
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clear accumulation of cells in mito
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the mice did not receive any doxycy
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113 Chromosome missegregations kill
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Chapter 6 Aneuploid tumor cells are
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Introduction Equal segregation of t
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S1A, data not shown). Based on thes
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A B U2OS + H2B-YFP percentage of ce
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tumor cells, leaving diploid cells
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A B p-S10-Histone H3 DAPI C µmol/l
Page 128 and 129:
Supplemental Figure-1. Protein bioc
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Bioavailability of compound-5 after
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A B U2OS (7.5nM) Hela (10nM) RPE-1
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7 Abstract Taxanes, such as docetax
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7 of mitotic aberrations. These dat
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7 A Low CFP lifetime High CFP lifet
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7 docetaxel treatment in vitro resu
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7 chromosome unstable, which means
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7 Cells (~50.000/well) were plated
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7 Supplemental data Supplemental fi
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7 148
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8 Thesis summary Unequal separation
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8 exerted through contraction of th
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8 1.4 NoCut: preventing the inducti
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8 tumorformation using intravital i
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Addendum References Nederlandse sam
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41. McEwen, B.F., et al., CENP-E is
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2010. 6(5): p. 359-68. 124. Liu, S.
Page 166 and 167:
210. Stucki, M., et al., MDC1 direc
Page 168 and 169:
tumors in mice. Cancer Res, 2007. 6
Page 170 and 171:
adiotherapy in breast cancer. Breas
Page 172 and 173:
470. Marcus, A.I., et al., Mitotic
Page 174 and 175:
559. Kienitz, A., et al., Partial d
Page 176 and 177:
Nederlandse Samenvatting Celdeling,
Page 178 and 179:
verdeling van tumorcellen compleet
Page 180 and 181:
Publications A. Janssen, R.H.Medema
Page 182 and 183:
En dan zijn er nu nog een aantal li
Page 184 and 185:
promotie-stukjes! Je bent een voorb
Page 186:
edankt voor al jullie hulp bij de i
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Chromosome segregation errors: a double-edged sword - TI Pharma

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