Chromosome segregation errors: a double-edged sword - TI Pharma

A
B
U2OS
(7.5nM)
Hela
(10nM)
RPE-1
(10nM)
% Apoptotic cells
30
25
20
15
10
5
0
- +
Compound-5
0 5 10 0 5 10 nM Taxol
0 1 2 0 1 2 nM Taxol
0 1 2 0 1 2 nM Taxol
Day 7
1 1/2
Dilution
1/4
C
Control
Mps1-WT
Mps1-602Q
DMSO
Taxol (5,2.5,1.25nM)
nM Compound-5
0 10 20 30 50
Compound-5 (100,50,25nM)
Taxol (5,2.5,1.25nM) + C5
(100nM)
Taxol (5,2.5,1.25nM) + C5
(50nM)
Taxol (5,2.5,1.25nM) + C5
(25nM)
Figure S2
A) Colony formation of U2OS, Hela and RPE-1 cells treated with indicated concentrations compound-5 (in between brackets) and
taxol. Taxol and compound-5 were added simultaneously and cells were harvested 6 days later. B) Graph depicting percentage of
apoptotic Hela cells 7 days after addition of indicated concentrations of compound-5 and taxol. Apoptosis was determined as the
number of propidium iodide positive cells over Hoechst positive nuclei. The highest concentration of each compound is indicated
with ‘dilution 1’ and the lowest with ‘dilution 1/4’. C) Colony formation assay of parental U2OS cells, U2OS cells stably expressing
Lap-Mps1-WT or Lap-Mps1-602Q in the presence of indicated concentrations compound-5.
131
Mps1 inhibition kills aneuploid cells
6