Addendum References Nederlandse samenvatting Curriculum Vitae Publications Dankwoord 159 Addendum &
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Howman, E.V., et al., Early disruption of centromeric chromatin organization in centromere protein A (Cenpa) null mice. Proc Natl Acad Sci U S A, 2000. 97(3): p. 1148-53. 15. Regnier, V., et al., CENP-A is required for accurate chromosome <strong>segregation</strong> and sustained kinetochore association of BubR1. Mol Cell Biol, 2005. 25(10): p. 3967-81. 16. Guse, A., M. Mishima, and M. Glotzer, Phosphorylation of ZEN-4/MKLP1 by aurora B regulates completion of cytokinesis. Curr Biol, 2005. 15(8): p. 778-86. 17. Heun, P., et al., Mislocalization of the Drosophila centromere-specific histone CID promotes formation of functional ectopic kinetochores. Dev Cell, 2006. 10(3): p. 303-15. 18. Blower, M.D., B.A. Sullivan, and G.H. Karpen, Conserved organization of centromeric chromatin in flies and humans. Dev Cell, 2002. 2(3): p. 319-30. 19. Cheeseman, I.M. and A. Desai, Molecular architecture of the kinetochore-microtubule interface. Nat Rev Mol Cell Biol, 2008. 9(1): p. 33-46. 20. 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Chromosome segregation errors: a do
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Chromosome segregation errors: a do
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Get up, stand up, stand up for your
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Chapter 1 General Introduction Anie
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cytoplasms and pinches off the memb
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which sister-chromatids are not pro
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Non-MCC members, but important mito
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completely separated. Following cle
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Two striking features of cancer cel
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predisposition at a very young age,
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that aberrant spindle formation inc
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(Fig.7C). As discussed in section 4
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Thesis outline Chromosome segregati
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286,395,407,408 Increase (thymus) I
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2 Abstract Various types of chromos
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2 34 A C D E % of cells Control 100
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2 assess whether cytokinesis induce
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2 A Asynchronous Monastrol release
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2 for 14 hours (unless indicated ot
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2 Immuno Blotting Cells were lysed
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2 Supplemental figures 44 A B C % o
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2 A MCF7 B C 53BP1 foci/cell 46 % o
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2 A siLuc siATM C 48 p-S1981 ATM me
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2 50 A B C % of EdU positive cells
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Chapter 3 Studying Chromosomal inst
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Introduction Aneuploidy is a common
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activity by ~80% 170-172 . With the
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A WT/CiMKi T649A Embryo’s: Figure
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control treated MEFs (unpaired t te
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Materials and Methods Cloning targe
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Primers: pGH_pA_FW#2: TCTATGGCTTCTG
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was prepared using standard procedu
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71 Cre-inducible Mps1 knock-in mous
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4 Abstract Most of the current drug
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4 Two other interesting mitotic tar
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4 inhibition of the anti-apoptotic
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4 cluster their centrosomes. This
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4 of checkpoint function can be ach
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4 4.5 Disadvantages of exploiting m
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Chapter 5 Elevating the frequency o
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Introduction Error-free chromosome
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Partial depletion of Mps1 or BubR1
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death is not induced in the short t
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had survived growth for 7 days in t
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The following antibodies have been
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Supplemental data Supplemental figu
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A BubR1 α-tubulin Hela-TetRBubR1 -
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- doxycycline + doxycycline - doxyc
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A B C percentage of cells % PI posi
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