Chromosome segregation errors: a double-edged sword - TI Pharma

7
Supplemental data
Supplemental figures
CFP/YFP ratio
146
A B
3
2
1
0
- +
H2B-D switch
CFP/YFP ratio
indicated. Average + SD is shown of 3 independent positions.
0.8
0.7
0.6
0.5
0.4
0
-10:00 0:00 1:00 2:00
H2B-D
C3-CAAX
A B
Fluorescence Lifetime (nsec)
2,0
1,5
1
0
C26-Day2
After Docetaxel
Inside Middle Imaged
Interphase
Mitosis
G1
-20 -15 -10 -5 0 1 2
CFP/YFP ratio
0.6
0.5
0.4
0.3
0.2
0.1
0
Time (hours)
Figure S1
A) Quantification of CFP/YFP ratio before and after H2B-D switching in vitro. n=10 cells per condition + SEM. B) Top: Representative
stills of a SW480 cell stably expressing H2B-D (red) and C3-CAAX (green) progressing through interphase and mitosis without
addition of drugs. Time in hours before mitosis is shown. T=0.00 indicates mitotic entry. Bottom: quantification of CFP-YFP ratio of
cells progressing through the cell cycle. n=11 cells. Average + SD is shown.
Interphase cells
- + Dtx
n=36 n=41
Figure S2
A) FLIM analysis of single cells (dots) in sections of indicated parts of the tumor. Tumor was isolated two days after docetaxel
treatment. ‘Inside’ indicates outer part of tumor (opposite of the imaging window site). ‘Middle’ indicates middle part of tumor
(cross section). ‘Imaged’ indicates part of tumor that was imaged through the imaging window. Each dot represents one cell. Line
indicates average + SD. B) Quantification of the CFP-YFP ratio of interphase SW480 cells in cell culture in the absence or presence
of taxanes. N indicates number of cells analyzed. Average + SD is shown.