Myeloid Leukemia

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Contributors xi CHRISTOPHE PICARD • Department of Biochemistry & Molecular Biology, Hôpital Universitaire Nord (AP-HM), ERT-MEIDIA-IFR Jean Roche- Université de la Méditerranée, Marseille, France VINCENZO ROSSI • Centro Ricerca M. Tettamanti, Clinica Pediatrica Università di Milano Bicocca and Dipartimento di Medicina Clinica, Prevenzione e Biotecnologie Sanitarie, Ospedale San Gerardo, Monza, Italy GIUSEPPE SAGLIO • Department of Clinical and Biological Sciences, University of Turin, Turin, Italy LINDA M. SCOTT • Department of Haematology, University of Cambridge, Cambridge, UK MIKE SCOTT • Department of Haematology, Addenbrookes NHS Trust, Cambridge, UK MONIQUE SILVY • Department of Biochemistry & Molecular Biology, Hôpital Universitaire Nord (AP-HM), ERT-MEIDIA-IFR Jean Roche-Université de la Méditerranée, Marseille, France KERRYN STONER • Department of Haematology, Addenbrookes NHS Trust, Cambridge, UK ELIZABETH H. STOVER • Brigham and Women’s Hospital, Harvard Medical School, Boston, MA LUCIANA TEOFILI • Instituti di Anatomia Patologica and Ematologia, Università Cattolica del Sacro Cuore, Roma, Italy KHALID TOBAL • The Rayne Institute, King’s College Hospital, London, United Kingdom ALISON V. TODD • Johnson & Johnson Research Pty. Limited, NSW, Australia BERT A. VAN DER REIJDEN • Central Hematology Laboratory, University Medical Center St. Raboud, Nijmegen, The Netherlands FRANZ WATZINGER • Children’s Cancer Research Institute (CCRI), Vienna, Austria ADRIAN WOOLFSON • University of Cambridge School of Clinical Medicine, Addenbrooke’s Hospital, Cambridge, United Kingdom JOHN A. LIU YIN • Hematology Department, University of Manchester, Manchester, UK
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Page 7 and 8: vi Preface comprehensive review of
Page 9 and 10: viii Contents 11 Detection of the F
Page 11: x Contributors D. GARY GILLILAND
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50 Picard, Silvy, and Gabert quanti
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52 Picard, Silvy, and Gabert sition
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56 Picard, Silvy, and Gabert and is
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58 Picard, Silvy, and Gabert tively
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60 Picard, Silvy, and Gabert qualit
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62 Picard, Silvy, and Gabert Fig. 1
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64 Picard, Silvy, and Gabert 6. Van
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66 Picard, Silvy, and Gabert 35. Sa
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68 Picard, Silvy, and Gabert 63. Ma
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70 Branford and Hughes selected pat
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72 Branford and Hughes The majority
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74 Branford and Hughes 14. Ampicill
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76 Branford and Hughes 4. Transfer
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78 Branford and Hughes 3.3.1. PCR 1
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80 Branford and Hughes cell lines t
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82 Branford and Hughes a. 1 cycle a
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84 Branford and Hughes 5. Pipet 22.
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88 Branford and Hughes Fig. 3. Caut
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90 Branford and Hughes 6. When the
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92 Branford and Hughes 25. Rubinste
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94 Branford and Hughes blast crisis
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96 Branford and Hughes kinase domai
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98 Branford and Hughes and reverse
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100 Branford and Hughes ries, add 2
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102 Branford and Hughes Fig. 2. Det
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104 Branford and Hughes from the sa
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106 Branford and Hughes and STI-571
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108 Campbell ABL fusion on der(22)
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110 Campbell Fig. 2. Fluorescent si
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112 Campbell 4. Notes 1. The diagno
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114 Campbell tion of DNA on the der
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116 Rossi, Levati, and Biondi Fig.
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118 Rossi, Levati, and Biondi assoc
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120 Rossi, Levati, and Biondi 2. Et
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126 Rossi, Levati, and Biondi 16. M
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128 Mokany et al. Key Words: QZyme
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130 Mokany et al. total RNA extract
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132 Mokany et al. 2.3. Duplex Singl
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134 Mokany et al. 3.2. Calibration
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136 Mokany et al. Table 1 Mutiplex
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Table 138 3 Mokany et al. Acceptanc
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140 Mokany et al. Table 4 Summary o
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142 Mokany et al. 4. Thermocycle th
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144 Mokany et al. or taken out of t
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146 Mokany et al. 21. It is possibl
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148 Mokany et al.
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150 Tobal and Yin 2. Materials 1. F
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152 Tobal and Yin 1. A negative (no
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154 Tobal and Yin two strategies fo
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156 Tobal and Yin Fig. 3. The TaqMa
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158 Tobal and Yin Fig. 5. Levels of
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160 Tobal and Yin 4. Notes 1. Some
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162 Tobal and Yin
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164 van der Reijden and Jansen tify
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166 van der Reijden and Jansen Fig.
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168 van der Reijden and Jansen Tabl
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170 van der Reijden and Jansen dete
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172 van der Reijden and Jansen CBFB
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174 van der Reijden and Jansen 10.
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176 van der Reijden and Jansen
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178 Cools, Stover, and Gilliland Fi
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180 Cools, Stover, and Gilliland ce
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182 Cools, Stover, and Gilliland Fi
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184 Cools, Stover, and Gilliland Ta
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186 Cools, Stover, and Gilliland 3.
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188 Cools, Stover, and Gilliland
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190 Kiyoi and Naoe most frequent ge
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192 Kiyoi and Naoe Table 1 Reaction
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194 Kiyoi and Naoe Fig. 1. Detectio
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196 Kiyoi and Naoe cell-lines have
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198 Kiyoi and Naoe
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200 Cilloni, Gottardi, and Saglio 1
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202 Cilloni, Gottardi, and Saglio M
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208 Cilloni, Gottardi, and Saglio F
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212 Cilloni, Gottardi, and Saglio
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214 Kohlmann et al. 2. Materials A
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216 Kohlmann et al. Fig. 1. Gene ex
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218 Kohlmann et al. sis procedure a
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220 Kohlmann et al. adding 175 µL
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222 Kohlmann et al. Table 5 Fragmen
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224 Kohlmann et al. available datab
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226 Kohlmann et al. 3.3.11. Quality
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228 Kohlmann et al. those patients
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230 Kohlmann et al. predictions for
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232 Kohlmann et al. unsupervised da
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234 Kohlmann et al. Fig. 5. Concept
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240 Kohlmann et al. 15. Jolliffe, I
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242 Christopherson et al. by most h
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244 Christopherson et al. 1.2.3. Di
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246 Christopherson et al. 4. Each b
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248 248 Christopherson et al.
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250 Christopherson et al. tiple sub
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252 Christopherson et al.
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254 Campbell et al. is not too diss
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256 Campbell et al. Fig. 1. Allele-
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258 Campbell et al. 2. Oligonucleot
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260 Campbell et al. a. 94°C for 11
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Table 1 Troubleshooting Allele-Spec
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264 Campbell et al. 8. Barosi, G.,
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266 Martini, Teofili, and Larocca r
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268 Martini, Teofili, and Larocca 3
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270 Martini, Teofili, and Larocca F
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272 Martini, Teofili, and Larocca R
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274 Martini, Teofili, and Larocca
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276 Lion and Watzinger rejection, p
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278 Lion and Watzinger 3. Flow-sort
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Table 1 Selected Microsatellite Loc
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282 Lion and Watzinger 7. Centrifug
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284 Lion and Watzinger Fig. 1. Exam
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286 Lion and Watzinger Fig. 3. Dete
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288 Lion and Watzinger from the don
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290 Lion and Watzinger Fig. 4. Nons
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292 Lion and Watzinger and thus com
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294 Lion and Watzinger References 1
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Index 297 Index A software, 236 ABL
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Index 299 CBFB-MYH11 fusion, breakp
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Index 301 materials, 257, 258, 260
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Index 303 AML1-ETO fusion monitorin
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Index 305 BCR-ABL fusion detection
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Myeloid Leukemia

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