Myeloid Leukemia

40 Picard, Silvy, and Gabert
Fig. 7. Specific fragment detection with hybridization probes. During the denaturation
step, both probes remain separate in solution. During the annealing step, the
probes hybridize and the two dyes come into close proximity. The energy emitted by
the excited probe is transferred to the second probe, which emits red fluorescent light
at a longer wavelength. At the polymerization step, both probes return into solution
and the fluorescence signal returns to background.
2.1.2.6. SCORPION PROBE
Scorpion probe/primer systems combine sequence-specific primers and
hybridization probes into a single structure, theoretically improving the kinetics
of fluorescent signal generation (26,27). Fig. 9A shows the basic elements
of a Scorpion probe. It consists of (1) a target-specific probe sequence held in
a hairpin loop configuration by complementary 5� and 3� arms, (2) a