Myeloid Leukemia

210 Cilloni, Gottardi, and Saglio
14. The threshold and baseline values have been established by the European network
in order to provide comparable results between different laboratories.
15. The software automatically generates standard curves for WT1 and ABL using
the mean Ct values of the different plasmid serial dilutions that have been
included in the plate. The mean Ct for both WT1 and ABL in each of the samples
is then automatically compared with the respective plasmid standard curves to
obtain the corresponding quantity (copy number) of transcripts in each sample
tested.
16. The final result expresses the WT1 copy number normalized per 10,000 ABL
copies.
17. The samples with an ABL Ct value >29 should be discarded, because the quality
of the sample is unacceptable.
References
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residual disease in childhood acute lymphoblastic leukemia. European Organization
for Research and Treatment of Cancer-Childhood Leukemia Cooperative
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WT1 expression by real time quantitative PCR may be a useful tool for monitoring
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8. Madden, S. L., Cook, D. M., Morris, J. F., Gashler, A., Sukhatme, V. P., and
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tumor gene product. Science 253, 1550–1553.
9. Park, S., Schalling, M., Bernard, A., et al. (1993) The Wilms tumor gene WT1 is
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