Myeloid Leukemia

Table 1
Characteristics of Different Instruments for Real-Time Polymerase Chain Reaction
Company Name Excitation Detection Wells Cycling system
Cepheid Smart Cycler Light-emitting diodes 4 channels (510–527, 16 I-CORE® modules
(LEDs)—4 channel 565–590, 606–650,
(450–495, 500–550, 670–750 nm)
565–590, 630–650 nm)
Idaho R.A.P.I.D. 450–490 nm 3 channels (520–540, 32 Fan & heating coil
Technology Inc. System 630–650,
690–730 nm)
Roche Applied LightCycler ® Blue LED light 3 channels (530 nm, 32 Heated air
Science (470 nm) 640 nm, 710 nm)
LightCycler ® 2.0 Blue LED light 6 channels (53, 560, 32 Heated air
(470 nm) 610, 640, 710 nm)
Corbett Research Rotor-Gene 3000 LED four excitation PMT 510, 555, 610, 36 & 72
filters (470, 530, 580 hp, 610 hp,
585, 625 nm) 660 hp nm
Applied Biosystems ABI prism 7000 Tungsten-halogen Four-position filter 96 Peltier
wheel and CCD
camera
ABI prism 7300 Single excitation Four emission filters 96 Peltier
CCD camera
ABI prism 7500 Five excitation Five emission filters 96 Peltier
CCD camera
ABI prism 7900 488 nm argon-ion laser 96/384 Peltier
Bio-Rad iCycler Tungsten-halogen lamp CCD 96 Peltier
(400–700 nm)
44 Picard, Silvy, and Gabert